Coherent X-ray diffractive imaging of protein crystals
The technique of coherent X-ray diffraction imaging (CXDI) has recently shown great promise for the study of inorganic nanocrystals. In this work the CXDI method has been applied to the study of micrometer-size protein crystals. Finely sampled diffraction patterns of single crystals were measured an...
| Veröffentlicht in: | Journal of synchrotron radiation. - 1994. - 15(2008), Pt 6 vom: 11. Nov., Seite 576-83 |
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| Format: | Online-Aufsatz |
| Sprache: | English |
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2008
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| Zugriff auf das übergeordnete Werk: | Journal of synchrotron radiation |
| Schlagworte: | Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Proteins |
| Zusammenfassung: | The technique of coherent X-ray diffraction imaging (CXDI) has recently shown great promise for the study of inorganic nanocrystals. In this work the CXDI method has been applied to the study of micrometer-size protein crystals. Finely sampled diffraction patterns of single crystals were measured and iterative phase-retrieval algorithms were used to reconstruct the two-dimensional shape of the crystal. The density maps have limited reproducibility because of radiation damage, but show clear evidence for crystal facets. Qualitative analysis of a number of single-crystal diffraction peaks indicates the presence of inward surface contraction on 2 microm size crystals. A survey of several hundred diffraction patterns yielded a number of examples with dramatic single-sided streaks, for which a plausible model is constructed |
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| Beschreibung: | Date Completed 08.01.2009 Date Revised 28.10.2008 published: Print-Electronic Citation Status MEDLINE |
| ISSN: | 1600-5775 |
| DOI: | 10.1107/S0909049508029439 |