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231223s2009 xx |||||o 00| ||eng c |
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|a 10.1016/j.jplph.2008.09.002
|2 doi
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|a pubmed24n0613.xml
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|a (DE-627)NLM183755367
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|a (NLM)18929428
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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| 100 |
1 |
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|a Kitazaki, Kazuyoshi
|e verfasserin
|4 aut
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| 245 |
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|a A mitochondrial gene involved in cytochrome c maturation (ccmC) is expressed as a precursor with a long NH2-terminal extension in sugar beet
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|c 2009
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| 336 |
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Completed 22.06.2009
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|a Date Revised 30.09.2020
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a Extensive genome rearrangement is one of the major mechanisms of angiosperm mitochondrial evolution. As a by-product, some angiosperm mitochondrial genes exhibit divergent organization, but not all of these genes have been fully characterized. Sugar beet ccmC, which plays an important role in cytochrome c maturation, harbors a unique extended NH(2) terminal region of 277 amino acid residues (N-extension) instead of a conserved translational initiation codon. The 5' termini of two major RNA species were determined by primer extension analysis, which revealed that the larger transcript covered the entire N-extension. Nucleotide sequencing of the cDNA revealed that a total of 31 C-to-U RNA editing events occurred in the N-extension and the ccmC-homologous region (ccmC-core region), resulting in improvement of amino acid sequence conservation. Antiserum was raised against a synthetic peptide corresponding to the ccmC-core region and was used for protein gel blot analysis of sugar beet and radish mitochondrial proteins. The detected 29.5-kDa signal band is shared by sugar beet and radish. Two additional larger signal bands are exclusively detected from sugar beet. The largest signal band is also detected by anti-N-extension antiserum. Our results indicate that sugar beet ccmC is translated as a long precursor with N-extension
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|a Journal Article
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| 650 |
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4 |
|a Research Support, Non-U.S. Gov't
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| 650 |
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7 |
|a DNA Primers
|2 NLM
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|a Peptides
|2 NLM
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| 650 |
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7 |
|a Plant Proteins
|2 NLM
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| 650 |
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|a Protein Precursors
|2 NLM
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| 650 |
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7 |
|a RNA, Messenger
|2 NLM
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| 700 |
1 |
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|a Nomoto, Yuta
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Aoshima, Akihiro
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Mikami, Tetsuo
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Kubo, Tomohiko
|e verfasserin
|4 aut
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| 773 |
0 |
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|i Enthalten in
|t Journal of plant physiology
|d 1979
|g 166(2009), 7 vom: 01. Mai, Seite 775-80
|w (DE-627)NLM098174622
|x 1618-1328
|7 nnns
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| 773 |
1 |
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|g volume:166
|g year:2009
|g number:7
|g day:01
|g month:05
|g pages:775-80
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| 856 |
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|u http://dx.doi.org/10.1016/j.jplph.2008.09.002
|3 Volltext
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|d 166
|j 2009
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|h 775-80
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