Angiotensin II type 2 receptor expression and its modulation in angiotensin II induced acute lung injury in rat
OBJECTIVE: To study the effect of angiotensin II (Ang II) and losartan, which is an angiotensin II type 1 receptor (AT1R) antagonist, on expression of AT2R in rat lung and the relationship between AT2R with acute lung injury (ALI)
| Veröffentlicht in: | Zhongguo wei zhong bing ji jiu yi xue = Chinese critical care medicine = Zhongguo weizhongbing jijiuyixue. - 1998. - 20(2008), 10 vom: 17. Okt., Seite 585-7 |
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| 1. Verfasser: | |
| Weitere Verfasser: | , , , , , |
| Format: | Aufsatz |
| Sprache: | Chinese |
| Veröffentlicht: |
2008
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| Zugriff auf das übergeordnete Werk: | Zhongguo wei zhong bing ji jiu yi xue = Chinese critical care medicine = Zhongguo weizhongbing jijiuyixue |
| Schlagworte: | English Abstract Journal Article Research Support, Non-U.S. Gov't Angiotensin II Type 1 Receptor Blockers RNA, Messenger Receptor, Angiotensin, Type 2 Angiotensin II 11128-99-7 Losartan JMS50MPO89 |
| Zusammenfassung: | OBJECTIVE: To study the effect of angiotensin II (Ang II) and losartan, which is an angiotensin II type 1 receptor (AT1R) antagonist, on expression of AT2R in rat lung and the relationship between AT2R with acute lung injury (ALI) METHODS: Twenty-four Sprague-Dawley (SD) rats were randomly divided into normal group, Ang II group, Ang II + losartan group and losartan group, with 6 rats in each group. Normal group and Ang II group were treated with continuous subcutaneous injection of normal saline (NS, 1 microg.kg(-1).min(-1)) or Ang II (1 microg.kg(-1).min(-1)) for 72 hours respectively. Ang II + losartan group was gavaged with losartan (10 mg.kg(-1) .d(-1)) 24 hours before and during the 72 hours' continuous subcutaneous injection of Ang II for a duration of 4 days. In losartan group rats were gavaged with losartan (10 mg.kg(-1).d(-1)) for 4 days only. Reverse transcription-polymerase chain reaction (RT-PCR) and Western blotting were employed to measure AT2R mRNA and protein. The pathology of the rat pulmonary was scored RESULTS: Smith's score of pathology in Ang II group [(3.33+/-1.14) scores] was significantly higher than that of the normal group [(0.73+/-0.09) scores], while it was significantly lower in Ang II + losartan group [(1.98+/-0.30) scores] than that of Ang II group. Smith's score of pathology in losartan group was [(0.95+/-0.20) scores]. The differences among four groups showed significant statistical difference (P<0.05 or P<0.01). AT2R mRNA in Ang II group [(47.90+/-9.88)%] was significantly lower than that of normal group [(86.33+/-5.90)%], while it was significantly higher in Ang II + losartan group [(90.63+/-19.66)%] than Ang II group . AT2R mRNA of losartan group was (68.65+/-4.88)% . The differences among four groups had significant statistical difference (P<0.05 or P<0.01). While on protein level showed no statistically significant difference among the four groups. The values of AT2R were (78.80+/- 41.26)%, (68.98+/-23.93)%, (68.13+/-23.23)% and (70.15+/-17.16)%, respectively CONCLUSION: Ang II could induce ALI and downregulate AT2R mRNA expression in the lung of ALI rats, whereas losartan acts as a positive regulator. AT2R seems to present lung protection in ALI |
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| Beschreibung: | Date Completed 09.03.2010 Date Revised 21.11.2013 published: Print Citation Status MEDLINE |
| ISSN: | 1003-0603 |