Monitoring helicase activity with molecular beacons
A high-throughput, fluorescence-based helicase assay using molecular beacons is described. The assay is tested using the NS3 helicase encoded by the hepatitis C virus (HCV) and is shown to accurately monitor helicase action on both DNA and RNA. In the assay, a ssDNA oligonucleotide molecular beacon,...
| Veröffentlicht in: | BioTechniques. - 1991. - 45(2008), 4 vom: 01. Okt., Seite 433-40, 442 |
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| Format: | Online-Aufsatz |
| Sprache: | English |
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2008
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| Zugriff auf das übergeordnete Werk: | BioTechniques |
| Schlagworte: | Journal Article Research Support, N.I.H., Extramural Carbocyanines NS3 protein, hepatitis C virus Viral Nonstructural Proteins cyanine dye 3 cyanine dye 5 RNA 63231-63-0 DNA |
| Online verfügbar |
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