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231223s2008 xx |||||o 00| ||eng c |
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|a 10.1093/jxb/ern241
|2 doi
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|a pubmed24n0610.xml
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|a (DE-627)NLM182846393
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|a (NLM)18836139
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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1 |
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|a Li, Hong-Ye
|e verfasserin
|4 aut
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|a Ethylene- and pathogen-inducible Arabidopsis acyl-CoA-binding protein 4 interacts with an ethylene-responsive element binding protein
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|c 2008
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Completed 15.12.2008
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|a Date Revised 09.03.2022
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a Six genes encode proteins with acyl-CoA-binding domains in Arabidopsis thaliana. They are the small 10-kDa cytosolic acyl-CoA-binding protein (ACBP), membrane-associated ACBP1 and ACBP2, extracellularly-targeted ACBP3, and kelch-motif containing ACBP4 and ACBP5. Here, the interaction of ACBP4 with an A. thaliana ethylene-responsive element binding protein (AtEBP), identified in a yeast two-hybrid screen, was confirmed by co-immunoprecipitation. The subcellular localization of ACBP4 and AtEBP, was addressed using an ACBP4:DsRed red fluorescent protein fusion and a green fluorescent protein (GFP):AtEBP fusion. Transient expression of these autofluoresence-tagged proteins in agroinfiltrated tobacco leaves, followed by confocal laser scanning microscopy, indicated their co-localization predominantly at the cytosol which was confirmed by FRET analysis. Immuno-electron microscopy on Arabidopsis sections not only localized ACBP4 to the cytosol but also to the periphery of the nucleus upon closer examination, perhaps as a result of its interaction with AtEBP. Furthermore, the expression of ACBP4 and AtEBP in Northern blot analyses was induced by the ethylene precursor 1-aminocyclopropane-1-carboxylic acid, methyl jasmonate treatments, and Botrytis cinerea infection, suggesting that the interaction of ACBP4 and AtEBP may be related to AtEBP-mediated defence possibly via ethylene and/or jasmonate signalling
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a ACBP4 protein, Arabidopsis
|2 NLM
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|a Arabidopsis Proteins
|2 NLM
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|a Carrier Proteins
|2 NLM
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|a DNA-Binding Proteins
|2 NLM
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|a Ethylenes
|2 NLM
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|a Plant Proteins
|2 NLM
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| 650 |
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|a ethylene-responsive element binding protein
|2 NLM
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| 650 |
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|a 164326-51-6
|2 NLM
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| 650 |
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|a ethylene
|2 NLM
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| 650 |
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|a 91GW059KN7
|2 NLM
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| 700 |
1 |
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|a Xiao, Shi
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Chye, Mee-Len
|e verfasserin
|4 aut
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| 773 |
0 |
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|i Enthalten in
|t Journal of experimental botany
|d 1985
|g 59(2008), 14 vom: 28., Seite 3997-4006
|w (DE-627)NLM098182706
|x 1460-2431
|7 nnns
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| 773 |
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|g volume:59
|g year:2008
|g number:14
|g day:28
|g pages:3997-4006
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|u http://dx.doi.org/10.1093/jxb/ern241
|3 Volltext
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