Kinetics of enzyme attack on substrates covalently attached to solid surfaces : influence of spacer chain length, immobilized substrate surface concentration and surface charge

The use of alpha-chymotrypsin to cleave covalently bound N-acetyl- l-tryptophan (Ac-Trp-OH) from the surfaces of aminopropylated controlled pore glass (CPG) and the polymer PEGA 1,900 was investigated. Oligoglycine spacer chains were used to present the covalently attached Ac-Trp-OH substrate to the...

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Détails bibliographiques
Publié dans:Langmuir : the ACS journal of surfaces and colloids. - 1991. - 24(2008), 20 vom: 21. Okt., Seite 11762-9
Auteur principal: Deere, Joseph (Auteur)
Autres auteurs: De Oliveira, Rui F, Tomaszewski, Bartłomiej, Millar, Sarah, Lalaouni, Antonia, Solares, Laura F, Flitsch, Sabine L, Halling, Peter J
Format: Article en ligne
Langue:English
Publié: 2008
Accès à la collection:Langmuir : the ACS journal of surfaces and colloids
Sujets:Journal Article Research Support, Non-U.S. Gov't Polymers bis(polyoxyethylene bis(amine)) 24991-53-5 Polyethylene Glycols 3WJQ0SDW1A Chymotrypsin EC 3.4.21.1 Glycine TE7660XO1C