Kinetics of enzyme attack on substrates covalently attached to solid surfaces : influence of spacer chain length, immobilized substrate surface concentration and surface charge
The use of alpha-chymotrypsin to cleave covalently bound N-acetyl- l-tryptophan (Ac-Trp-OH) from the surfaces of aminopropylated controlled pore glass (CPG) and the polymer PEGA 1,900 was investigated. Oligoglycine spacer chains were used to present the covalently attached Ac-Trp-OH substrate to the...
Ausführliche Beschreibung
Bibliographische Detailangaben
| Veröffentlicht in: | Langmuir : the ACS journal of surfaces and colloids. - 1991. - 24(2008), 20 vom: 21. Okt., Seite 11762-9
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| 1. Verfasser: |
Deere, Joseph
(VerfasserIn) |
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| Weitere Verfasser: |
De Oliveira, Rui F,
Tomaszewski, Bartłomiej,
Millar, Sarah,
Lalaouni, Antonia,
Solares, Laura F,
Flitsch, Sabine L,
Halling, Peter J |
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| Format: | Online-Aufsatz
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| Sprache: | English |
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| Veröffentlicht: |
2008
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| Zugriff auf das übergeordnete Werk: | Langmuir : the ACS journal of surfaces and colloids
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| Schlagworte: | Journal Article
Research Support, Non-U.S. Gov't
Polymers
bis(polyoxyethylene bis(amine))
24991-53-5
Polyethylene Glycols
3WJQ0SDW1A
Chymotrypsin
EC 3.4.21.1
Glycine
TE7660XO1C |
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