Entrapping desired amounts of actin filaments and molecular motor proteins in giant liposomes
We have successfully prepared cell-sized giant liposomes encapsulating desired amounts of actoHMM, a mixture of actin filament (F-actin) and heavy meromyosin (HMM, an actin-related molecular motor), in the presence of 5 mM MgCl 2 and 50 mM KCl. We employed a spontaneous transfer method to prepare th...
Ausführliche Beschreibung
Bibliographische Detailangaben
| Veröffentlicht in: | Langmuir : the ACS journal of surfaces and colloids. - 1992. - 24(2008), 20 vom: 21. Okt., Seite 11323-6
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| 1. Verfasser: |
Takiguchi, Kingo
(VerfasserIn) |
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| Weitere Verfasser: |
Yamada, Ayako,
Negishi, Makiko,
Tanaka-Takiguchi, Yohko,
Yoshikawa, Kenichi |
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| Format: | Online-Aufsatz
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| Sprache: | English |
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| Veröffentlicht: |
2008
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| Zugriff auf das übergeordnete Werk: | Langmuir : the ACS journal of surfaces and colloids
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| Schlagworte: | Journal Article
Research Support, Non-U.S. Gov't
Actins
Liposomes
Molecular Motor Proteins
Myosins
EC 3.6.4.1 |
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