Application of the rpoS gene for the detection of Vibrio anguillarum in flounder and prawn by polymerase chain reaction
Vibrio anguillarum, an opportunistic fish pathogen, is the main species responsible for vibriosis, a disease that affects feral and farmed fish and shellfish, and causes considerable economic losses in marine aquaculture. In this study, we used polymerase chain reaction (PCR) to detect V. anguillaru...
| Veröffentlicht in: | Journal of fish diseases. - 1998. - 31(2008), 9 vom: 01. Sept., Seite 639-47 |
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| 1. Verfasser: | |
| Weitere Verfasser: | , , , , |
| Format: | Online-Aufsatz |
| Sprache: | English |
| Veröffentlicht: |
2008
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| Zugriff auf das übergeordnete Werk: | Journal of fish diseases |
| Schlagworte: | Journal Article Research Support, Non-U.S. Gov't Bacterial Proteins Sigma Factor sigma factor KatF protein, Bacteria |
| Zusammenfassung: | Vibrio anguillarum, an opportunistic fish pathogen, is the main species responsible for vibriosis, a disease that affects feral and farmed fish and shellfish, and causes considerable economic losses in marine aquaculture. In this study, we used polymerase chain reaction (PCR) to detect V. anguillarum. PCR specificity was evaluated by amplifying the rpoS gene, a general stress regulator, in six strains of V. anguillarum and 36 other bacterial species. PCR amplified a species-specific fragment (689 bp) from V. anguillarum. Furthermore, the PCR assay was sensitive enough to detect rpoS expression from 3 pg of genomic DNA, or from six colony-forming units (CFU) mL(-1) of cultured V. anguillarum. However, the assay was less sensitive when genomic DNA from the infected flounder and prawn was used (limit of detection, 50 ng and 10 ng g(-1) tissue, respectively). These data demonstrate that PCR amplification of the rpoS gene is a sensitive and species-specific method to detect V. anguillarum in practical situations |
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| Beschreibung: | Date Completed 18.12.2008 Date Revised 30.09.2020 published: Print GENBANK: AY695434 Citation Status MEDLINE |
| ISSN: | 1365-2761 |
| DOI: | 10.1111/j.1365-2761.2008.00943.x |