Control of the compaction/unfolding transition of genomic DNA by the addition/disruption of lipid assemblies

We studied the interaction between individual long genomic DNA molecules and cationic lipid assemblies. The assembly of cationic lipid molecules into small unilamellar vesicles (SUVs) of about 50 nm diameter led to the compaction of DNA whereas the addition of a neutral surfactant resulted in the di...

Ausführliche Beschreibung

Bibliographische Detailangaben
Veröffentlicht in:Langmuir : the ACS journal of surfaces and colloids. - 1992. - 24(2008), 19 vom: 07. Okt., Seite 10604-7
1. Verfasser: Diguet, Antoine (VerfasserIn)
Weitere Verfasser: Baigl, Damien
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2008
Zugriff auf das übergeordnete Werk:Langmuir : the ACS journal of surfaces and colloids
Schlagworte:Journal Article Research Support, Non-U.S. Gov't DNA, Viral Lipids
Beschreibung
Zusammenfassung:We studied the interaction between individual long genomic DNA molecules and cationic lipid assemblies. The assembly of cationic lipid molecules into small unilamellar vesicles (SUVs) of about 50 nm diameter led to the compaction of DNA whereas the addition of a neutral surfactant resulted in the disruption of SUVs and the unfolding of DNA. This reversible process does not require any chemical reaction or change in the ionic strength of the solution. It was applied to switch DNA repeatedly between a compact and an unfolded conformation in a dynamic manner
Beschreibung:Date Completed 28.10.2008
Date Revised 01.10.2008
published: Print-Electronic
Citation Status MEDLINE
ISSN:1520-5827
DOI:10.1021/la802317e