Plasmid sonication improves sequencing efficiency and quality in the Beckman Coulter CEQ system
We report on an unexpectedly high rate of unreadable chromatograms from plasmid sequencing using Beckman Coulter's protocols, chemistry, and CEQ8000 instrument. Failed or poor quality plasmid sequence chromatograms were accompanied by a sharp drop, fluctuation, or steady decline in the current...
Ausführliche Beschreibung
Bibliographische Detailangaben
| Veröffentlicht in: | BioTechniques. - 1991. - 45(2008), 3 vom: 01. Sept., Seite 327-9
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| 1. Verfasser: |
Thompson, Mark
(VerfasserIn) |
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| Weitere Verfasser: |
Aukema, Kelly,
O'Bryan, Dana,
Rader, Stephen,
Murray, Brent |
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| Format: | Online-Aufsatz
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| Sprache: | English |
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| Veröffentlicht: |
2008
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| Zugriff auf das übergeordnete Werk: | BioTechniques
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| Schlagworte: | Journal Article
Research Support, Non-U.S. Gov't
DNA, Superhelical
DNA
9007-49-2 |
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