A low-cost flow cytometric assay for the detection and quantification of apoptosis using an anionic halogenated fluorescein dye

We describe here a technical improvement of an established colorimetric method used to detect and measure the occurrence of apoptosis in mammalian cells during in vitro cell culture. This assay uses an anionic halogenated fluorescein dye that is taken up by apoptotic cells at the stage of phosphatid...

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Détails bibliographiques
Publié dans:BioTechniques. - 1991. - 45(2008), 3 vom: 01. Sept., Seite 317-20
Auteur principal: Meyer, Mervin (Auteur)
Autres auteurs: Essack, Magbubah, Kanyanda, Stonard, Rees, Jasper
Format: Article en ligne
Langue:English
Publié: 2008
Accès à la collection:BioTechniques
Sujets:Comparative Study Journal Article Research Support, Non-U.S. Gov't Annexin A5 Coloring Agents Fluorescent Dyes Fluorescein TPY09G7XIR