Design of a single plasmid-based modified yeast one-hybrid system for investigation of in vivo protein-protein and protein-DNA interactions
We have developed a modified yeast one-hybrid system (MY1H) useful for in vivo investigation of protein-protein and protein-DNA interactions. Our single-plasmid expression system is capable of differential protein expression levels; in addition to a GAL4 activation domain (AD) fusion protein, a seco...
Ausführliche Beschreibung
Bibliographische Detailangaben
| Veröffentlicht in: | BioTechniques. - 1993. - 45(2008), 3 vom: 01. Sept., Seite 295-304
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| 1. Verfasser: |
Chen, Gang
(VerfasserIn) |
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| Weitere Verfasser: |
DenBoer, Lisa,
Shin, Jumi |
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| Format: | Online-Aufsatz
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| Sprache: | English |
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| Veröffentlicht: |
2008
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| Zugriff auf das übergeordnete Werk: | BioTechniques
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| Schlagworte: | Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Antigens, Polyomavirus Transforming
Apoptosis Regulatory Proteins
Carrier Proteins
DNA-Binding Proteins
GAL4 protein, S cerevisiae
Lectins
Proteins
mehr...
Recombinant Fusion Proteins
Saccharomyces cerevisiae Proteins
TP53BP2 protein, human
Transcription Factors
Tumor Suppressor Protein p53
fucose-binding lectin
DNA
9007-49-2 |
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