Real-time PCR to determine transgene copy number and to quantitate the biolocalization of adoptively transferred cells from EGFP-transgenic mice

Quantitative real-time PCR (qPCR) is a sensitive technique for the detection and quantitation of specific DNA sequences. Here we describe a Taqman qPCR assay for quantification of tissue-localized, adoptively transferred enhanced green fluorescent protein (EGFP)-transgenic cells. A standard curve co...

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Bibliographische Detailangaben

Veröffentlicht in:	BioTechniques. - 1993. - 45(2008), 3 vom: 01. Sept., Seite 247-58
1. Verfasser:	Joshi, Molishree (VerfasserIn)
Weitere Verfasser:	Keith Pittman, H, Haisch, Carl, Verbanac, Kathryn
Format:	Online-Aufsatz
Sprache:	English
Veröffentlicht:	2008
Zugriff auf das übergeordnete Werk:	BioTechniques
Schlagworte:	Journal Article DNA, Neoplasm enhanced green fluorescent protein Green Fluorescent Proteins 147336-22-9 Taq Polymerase EC 2.7.7.-