Real-time PCR to determine transgene copy number and to quantitate the biolocalization of adoptively transferred cells from EGFP-transgenic mice

Quantitative real-time PCR (qPCR) is a sensitive technique for the detection and quantitation of specific DNA sequences. Here we describe a Taqman qPCR assay for quantification of tissue-localized, adoptively transferred enhanced green fluorescent protein (EGFP)-transgenic cells. A standard curve co...

Description complète

Détails bibliographiques

Publié dans:	BioTechniques. - 1991. - 45(2008), 3 vom: 01. Sept., Seite 247-58
Auteur principal:	Joshi, Molishree (Auteur)
Autres auteurs:	Keith Pittman, H, Haisch, Carl, Verbanac, Kathryn
Format:	Article en ligne
Langue:	English
Publié:	2008
Accès à la collection:	BioTechniques
Sujets:	Journal Article DNA, Neoplasm enhanced green fluorescent protein Green Fluorescent Proteins 147336-22-9 Taq Polymerase EC 2.7.7.-