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231223s2008 xx |||||o 00| ||eng c |
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|a 10.1093/jxb/ern212
|2 doi
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|a pubmed24n0606.xml
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|a (DE-627)NLM181777282
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|a (NLM)18723758
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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|a Aluru, Maneesha
|e verfasserin
|4 aut
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|a Generation of transgenic maize with enhanced provitamin A content
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|c 2008
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|a Text
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|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
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|2 rdacarrier
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|a Date Completed 24.11.2008
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|a Date Revised 20.10.2021
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a Vitamin A deficiency (VAD) affects over 250 million people worldwide and is one of the most prevalent nutritional deficiencies in developing countries, resulting in significant socio-economic losses. Provitamin A carotenoids such as beta-carotene, are derived from plant foods and are a major source of vitamin A for the majority of the world's population. Several years of intense research has resulted in the production of 'Golden Rice 2' which contains sufficiently high levels of provitamin A carotenoids to combat VAD. In this report, the focus is on the generation of transgenic maize with enhanced provitamin A content in their kernels. Overexpression of the bacterial genes crtB (for phytoene synthase) and crtI (for the four desaturation steps of the carotenoid pathway catalysed by phytoene desaturase and zeta-carotene desaturase in plants), under the control of a 'super gamma-zein promoter' for endosperm-specific expression, resulted in an increase of total carotenoids of up to 34-fold with a preferential accumulation of beta-carotene in the maize endosperm. The levels attained approach those estimated to have a significant impact on the nutritional status of target populations in developing countries. The high beta-carotene trait was found to be reproducible over at least four generations. Gene expression analyses suggest that increased accumulation of beta-carotene is due to an up-regulation of the endogenous lycopene beta-cylase. These experiments set the stage for the design of transgenic approaches to generate provitamin A-rich maize that will help alleviate VAD
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Research Support, U.S. Gov't, Non-P.H.S.
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|a Bacterial Proteins
|2 NLM
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|a beta Carotene
|2 NLM
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|a 01YAE03M7J
|2 NLM
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|a Alkyl and Aryl Transferases
|2 NLM
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|a EC 2.5.-
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|a Geranylgeranyl-Diphosphate Geranylgeranyltransferase
|2 NLM
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| 650 |
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|a EC 2.5.1.32
|2 NLM
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| 700 |
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|a Xu, Yang
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Guo, Rong
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Wang, Zhenguo
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Li, Shanshan
|e verfasserin
|4 aut
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| 700 |
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|a White, Wendy
|e verfasserin
|4 aut
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|a Wang, Kan
|e verfasserin
|4 aut
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| 700 |
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|a Rodermel, Steve
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Journal of experimental botany
|d 1985
|g 59(2008), 13 vom: 15., Seite 3551-62
|w (DE-627)NLM098182706
|x 1460-2431
|7 nnns
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| 773 |
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|g volume:59
|g year:2008
|g number:13
|g day:15
|g pages:3551-62
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|u http://dx.doi.org/10.1093/jxb/ern212
|3 Volltext
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