Percoll-purified and photosynthetically active chloroplasts from Arabidopsis thaliana leaves
The availability of the complete genome sequence of Arabidopsis thaliana and of large collections of insertion mutants paved the way for systematic studies of gene functions in this organism, thus requiring adapting biochemical and physiological tools to this model plant. For physiological analysis...
| Veröffentlicht in: | Plant physiology and biochemistry : PPB. - 1991. - 46(2008), 11 vom: 08. Nov., Seite 951-5 |
|---|---|
| 1. Verfasser: | |
| Weitere Verfasser: | , , , |
| Format: | Online-Aufsatz |
| Sprache: | English |
| Veröffentlicht: |
2008
|
| Zugriff auf das übergeordnete Werk: | Plant physiology and biochemistry : PPB |
| Schlagworte: | Journal Article Percoll 65455-52-9 Silicon Dioxide 7631-86-9 Povidone FZ989GH94E Oxygen S88TT14065 |
| Zusammenfassung: | The availability of the complete genome sequence of Arabidopsis thaliana and of large collections of insertion mutants paved the way for systematic studies of gene functions in this organism, thus requiring adapting biochemical and physiological tools to this model plant. For physiological analysis of photosynthesis, methods combining high level of chloroplast purity and preservation of the photosynthetic activity were missing. Here, we describe a rapid method (less than 1h) to obtain Percoll-purified and photosynthetically active chloroplasts from Arabidopsis leaves retaining almost 90% of the Vmax of photosynthesis measured in the starting leaves from plants grown under a light intensity of 150mumolphotonm(-2)s(-1) and 80% of their initial photosynthetic rate after 3h of storage |
|---|---|
| Beschreibung: | Date Completed 10.12.2008 Date Revised 21.03.2022 published: Print-Electronic Citation Status MEDLINE |
| ISSN: | 1873-2690 |
| DOI: | 10.1016/j.plaphy.2008.06.009 |