An isoform of Arabidopsis myosin XI interacts with small GTPases in its C-terminal tail region

Myosin XI, a class of myosins expressed in plants is believed to be responsible for cytoplasmic streaming and the translocation of organelles and vesicles. To gain further insight into the translocation of organelles and vesicles by myosin XI, an isoform of Arabidopsis myosin XI, MYA2, was chosen an...

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Bibliographische Detailangaben
Veröffentlicht in:	Journal of experimental botany. - 1985. - 59(2008), 13 vom: 15., Seite 3523-31
1. Verfasser:	Hashimoto, Kohsuke (VerfasserIn)
Weitere Verfasser:	Igarashi, Hisako, Mano, Shoji, Takenaka, Chikako, Shiina, Takashi, Yamaguchi, Masatoshi, Demura, Taku, Nishimura, Mikio, Shimmen, Teruo, Yokota, Etsuo
Format:	Online-Aufsatz
Sprache:	English
Veröffentlicht:	2008
Zugriff auf das übergeordnete Werk:	Journal of experimental botany
Schlagworte:	Journal Article Research Support, Non-U.S. Gov't Arabidopsis Proteins MYA2 protein, Arabidopsis Protein Isoforms RabD1 protein, Arabidopsis EC 3.6.1.- Myosin Heavy Chains EC 3.6.4.1 Monomeric GTP-Binding Proteins mehr... EC 3.6.5.2 RAC2 protein, Arabidopsis

Beschreibung
Zusammenfassung:	Myosin XI, a class of myosins expressed in plants is believed to be responsible for cytoplasmic streaming and the translocation of organelles and vesicles. To gain further insight into the translocation of organelles and vesicles by myosin XI, an isoform of Arabidopsis myosin XI, MYA2, was chosen and its role in peroxisome targeting was examined. Using the yeast two-hybrid screening method, two small GTPases, AtRabD1 and AtRabC2a, were identified as factors that interact with the C-terminal tail region of MYA2. Both recombinant AtRabs tagged with His bound to the recombinant C-terminal tail region of MYA2 tagged with GST in a GTP-dependent manner. Furthermore, AtRabC2a was localized on peroxisomes, when its CFP-tagged form was expressed transiently in protoplasts prepared from Arabidopsis leaf tissue. It is suggested that MYA2 targets the peroxisome through an interaction with AtRabC2a
Beschreibung:	Date Completed 24.11.2008 Date Revised 20.10.2021 published: Print-Electronic Citation Status MEDLINE
ISSN:	1460-2431
DOI:	10.1093/jxb/ern202