|
|
|
|
| LEADER |
01000naa a22002652 4500 |
| 001 |
NLM18157974X |
| 003 |
DE-627 |
| 005 |
20231223161508.0 |
| 007 |
cr uuu---uuuuu |
| 008 |
231223s2008 xx |||||o 00| ||eng c |
| 024 |
7 |
|
|a 10.1021/la801256h
|2 doi
|
| 028 |
5 |
2 |
|a pubmed24n0605.xml
|
| 035 |
|
|
|a (DE-627)NLM18157974X
|
| 035 |
|
|
|a (NLM)18702475
|
| 040 |
|
|
|a DE-627
|b ger
|c DE-627
|e rakwb
|
| 041 |
|
|
|a eng
|
| 100 |
1 |
|
|a Organero, Juan Angel
|e verfasserin
|4 aut
|
| 245 |
1 |
0 |
|a Chemical and biological caging effects on the relaxation of a proton-transfer dye
|
| 264 |
|
1 |
|c 2008
|
| 336 |
|
|
|a Text
|b txt
|2 rdacontent
|
| 337 |
|
|
|a ƒaComputermedien
|b c
|2 rdamedia
|
| 338 |
|
|
|a ƒa Online-Ressource
|b cr
|2 rdacarrier
|
| 500 |
|
|
|a Date Completed 17.10.2008
|
| 500 |
|
|
|a Date Revised 21.11.2013
|
| 500 |
|
|
|a published: Print-Electronic
|
| 500 |
|
|
|a Citation Status MEDLINE
|
| 520 |
|
|
|a We report studies of the interaction between a proton-transfer dye (1'-hydroxy,2'-acetonaphthone, HAN), with the human serum albumin (HSA) protein and a beta-cyclodextrin derivative (DM-beta-CD) in neutral water solutions. We used steady-state and picosecond time-resolved emission spectroscopy to follow the structural changes of HAN due to the hydrophobicity and confinement effect of these nanocavities. Upon encapsulation, the fluorescence intensity of the 1:1 inclusion complex in both cavities increases, and the emission lifetimes become longer. For the DM-beta-CD complexes, we obtained 430 and 920 ps, whereas for the HSA complexes we obtained 630 ps and 2 ns. Picosecond anisotropy measurements show strong confinement due to protein docking. The rotational time for the CD complex is 660 ps, whereas for the protein complex we find 6 ns. The process of energy transfer from the excited triptophan 214 (Trp214) of HSA to the trapped HAN occurs with high efficiency (71%), and the calculated distance between both chromophores is 17 A. We believe that the results are important for a better understanding of the processes occurring in inclusion complexes such as those in nanopharmacodynamics
|
| 650 |
|
4 |
|a Journal Article
|
| 650 |
|
4 |
|a Research Support, Non-U.S. Gov't
|
| 650 |
|
7 |
|a Coloring Agents
|2 NLM
|
| 650 |
|
7 |
|a Protons
|2 NLM
|
| 650 |
|
7 |
|a Serum Albumin
|2 NLM
|
| 650 |
|
7 |
|a beta-Cyclodextrins
|2 NLM
|
| 650 |
|
7 |
|a Water
|2 NLM
|
| 650 |
|
7 |
|a 059QF0KO0R
|2 NLM
|
| 650 |
|
7 |
|a betadex
|2 NLM
|
| 650 |
|
7 |
|a JV039JZZ3A
|2 NLM
|
| 700 |
1 |
|
|a Martin, Cristina
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Cohen, Boiko
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Douhal, Abderrazzak
|e verfasserin
|4 aut
|
| 773 |
0 |
8 |
|i Enthalten in
|t Langmuir : the ACS journal of surfaces and colloids
|d 1992
|g 24(2008), 18 vom: 16. Sept., Seite 10352-7
|w (DE-627)NLM098181009
|x 1520-5827
|7 nnns
|
| 773 |
1 |
8 |
|g volume:24
|g year:2008
|g number:18
|g day:16
|g month:09
|g pages:10352-7
|
| 856 |
4 |
0 |
|u http://dx.doi.org/10.1021/la801256h
|3 Volltext
|
| 912 |
|
|
|a GBV_USEFLAG_A
|
| 912 |
|
|
|a SYSFLAG_A
|
| 912 |
|
|
|a GBV_NLM
|
| 912 |
|
|
|a GBV_ILN_22
|
| 912 |
|
|
|a GBV_ILN_350
|
| 912 |
|
|
|a GBV_ILN_721
|
| 951 |
|
|
|a AR
|
| 952 |
|
|
|d 24
|j 2008
|e 18
|b 16
|c 09
|h 10352-7
|