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231223s2008 xx ||||| 00| ||chi c |
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|a (DE-627)NLM181440490
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|a (NLM)18687171
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|a DE-627
|b ger
|c DE-627
|e rakwb
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| 041 |
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|a chi
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| 100 |
1 |
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|a Bi, Ming-hua
|e verfasserin
|4 aut
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| 245 |
1 |
4 |
|a The effect of recombinant interleukin-10/Fc fusion protein on lipopolysaccharide-induced acute lung injury in mice
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| 264 |
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|c 2008
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| 336 |
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|a Text
|b txt
|2 rdacontent
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| 337 |
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|a ohne Hilfsmittel zu benutzen
|b n
|2 rdamedia
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| 338 |
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|a Band
|b nc
|2 rdacarrier
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| 500 |
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|a Date Completed 02.02.2010
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|a Date Revised 08.08.2008
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|a published: Print
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|a Citation Status MEDLINE
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| 520 |
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|a OBJECTIVE: To clarify the regulatory role and mechanism of recombinant interleukin-10/Fc (rIL-10/Fc) fusion protein on inflammatory parameters during development of acute lung injury (ALI) induced by lipopolysaccharide (LPS) in a murine model
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| 520 |
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|a METHODS: An ALI model was reproduced by intra-tracheal injection of LPS. rIL-10/Fc was administered intraperitoneally. One hundred and thirty-two BALB/c mice were divided into four groups, including saline control group, rIL-10/Fc control group, ALI model group, and rIL-10/Fc treatment group. Twenty-four-hour survival rate was determined in 25 mice of each group. The number of inflammatory cells and inflammatory mediators in bronchial-alveolar lavage fluid (BALF), tumor necrosis factor-alpha (TNF-alpha) and IL-1 beta, and also lung myeloperoxidase (MPO) activity, lung wet/dry (W/D) ratio were determined in the rest of mice. Pathological changes in lung were examined with hematoxylin-eosin (HE) staining, and inflammatory change was evaluated under microscope
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|a RESULTS: Levels of TNF-alpha and IL-1 beta in BALF were substantially increased 4 hours after intra-tracheal LPS (both P<0.01), and they were lowered but without significant difference after rIL-10/Fc administration. However, rIL-10/Fc fusion protein markedly attenuated release of TNF-alpha at 8 hours and 12 hours, and IL-1 beta was lowered at 12 hours after LPS challenge. Pre-treatment with rIL-10/Fc fusion protein significantly improved survival rate at 24 hours in LPS challenged mice (P<0.01). There was no significant difference in cell count in BALF, MPO, lung W/D ratio, after treatment of rIL-10/Fc fusion protein. Obvious inflammatory changes were found in lung was found pathologically at 24 hours after LPS injection, but there was no significant difference compared with ALI mice with rIL-10/Fc fusion protein administration
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| 520 |
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|a CONCLUSION: rIL-10/Fc fusion protein inhibits release of TNF-alpha and IL-1 beta in BALF in a LPS-induced ALI murine model. rIL-10/Fc fusion protein improves survival rate in ALI mice by decreasing the release of pro-inflammatory cytokines
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| 650 |
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|a English Abstract
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|a Journal Article
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| 650 |
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|a Research Support, Non-U.S. Gov't
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| 650 |
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7 |
|a Interleukin-1beta
|2 NLM
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| 650 |
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7 |
|a Lipopolysaccharides
|2 NLM
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| 650 |
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7 |
|a Recombinant Fusion Proteins
|2 NLM
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| 650 |
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7 |
|a Tumor Necrosis Factor-alpha
|2 NLM
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| 650 |
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7 |
|a Interleukin-10
|2 NLM
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| 650 |
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7 |
|a 130068-27-8
|2 NLM
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| 650 |
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7 |
|a Peroxidase
|2 NLM
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| 650 |
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7 |
|a EC 1.11.1.7
|2 NLM
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| 700 |
1 |
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|a Wang, Bao-en
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Zheng, Xin-xiao
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Li, Min
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Mayer, Konstantin
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Zhang, Shu-wen
|e verfasserin
|4 aut
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| 773 |
0 |
8 |
|i Enthalten in
|t Zhongguo wei zhong bing ji jiu yi xue = Chinese critical care medicine = Zhongguo weizhongbing jijiuyixue
|d 1998
|g 20(2008), 8 vom: 08. Aug., Seite 461-4
|w (DE-627)NLM098227793
|x 1003-0603
|7 nnns
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| 773 |
1 |
8 |
|g volume:20
|g year:2008
|g number:8
|g day:08
|g month:08
|g pages:461-4
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| 912 |
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|a SYSFLAG_A
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|a GBV_ILN_350
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| 951 |
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|a AR
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| 952 |
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|d 20
|j 2008
|e 8
|b 08
|c 08
|h 461-4
|