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231223s2008 xx |||||o 00| ||eng c |
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|a 10.1021/la801851h
|2 doi
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|a pubmed25n0605.xml
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|a (DE-627)NLM18143878X
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|a (NLM)18686976
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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| 100 |
1 |
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|a Li, Bin
|e verfasserin
|4 aut
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| 245 |
1 |
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|a Microscopic investigations of the Cr(VI) uptake mechanism of living Ochrobactrum anthropi
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| 264 |
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|c 2008
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| 336 |
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|a Text
|b txt
|2 rdacontent
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| 337 |
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|a ƒaComputermedien
|b c
|2 rdamedia
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| 338 |
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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| 500 |
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|a Date Completed 13.11.2008
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|a Date Revised 21.11.2013
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a A basic understanding related to the immobilization of chromium by bacteria is essential for chromate pollutant remediation in the environment. In this work, we studied the Cr(VI) uptake mechanism of living Ochrobactrum anthropi and the influence of a bacterial culture medium on the Cr-immobilization process. It was found that the Cr-immobilization ratio of bacteria in Tris-HCl buffer is higher than in LB medium. X-ray photoelectron spectroscopy (XPS) and electron paramagnetic resonance (EPR) analysis revealed that the chromium accumulated on bacteria were mostly in Cr(III) states. Scanning electron microscopy (SEM) and atomic force microscopy (AFM) observations showed that noticeable Cr(III) precipitates were accumulated on bacterial surfaces. AFM roughness analysis revealed that the surface roughness of bacteria increased greatly when the bacteria-Cr(VI) interaction was in Tris-HCl buffer rather than in LB solution. Transmission electron microscopy (TEM) thin section analysis coupled with energy-dispersive X-ray spectroscopy showed that Cr(III) is also distributed in bacterial inner portions. A chromium-immobilization mechanism considering the participation of both bacterial inner portions and bacterial surfaces of living Ochrobactrum anthropi was proposed, whereas the bacterial surface was the dominant part of the immobilization of Cr(III). This work also proved that the control of Cr immobilization by living Ochrobactrum anthropi could be achieved via adjusting the bacterial culture medium
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Carcinogens, Environmental
|2 NLM
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|a Chromates
|2 NLM
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|a Culture Media
|2 NLM
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|a Chromium
|2 NLM
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| 650 |
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7 |
|a 0R0008Q3JB
|2 NLM
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| 700 |
1 |
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|a Pan, Danmei
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Zheng, Jinsheng
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Cheng, Yangjian
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Ma, Xiaoyan
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Huang, Feng
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Lin, Zhang
|e verfasserin
|4 aut
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| 773 |
0 |
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|i Enthalten in
|t Langmuir : the ACS journal of surfaces and colloids
|d 1991
|g 24(2008), 17 vom: 02. Sept., Seite 9630-5
|w (DE-627)NLM098181009
|x 0743-7463
|7 nnns
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| 773 |
1 |
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|g volume:24
|g year:2008
|g number:17
|g day:02
|g month:09
|g pages:9630-5
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|u http://dx.doi.org/10.1021/la801851h
|3 Volltext
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