Root uptake of cationic amino acids by Arabidopsis depends on functional expression of amino acid permease 5

* Specific transporters mediate uptake of amino acids by plant roots. Earlier studies have indicated that the lysine histidine transporter 1 and amino acid permease 1 participate in this process, but although plant roots have been shown to absorb cationic amino acids with high affinity, neither of t...

Ausführliche Beschreibung

Bibliographische Detailangaben
Veröffentlicht in:The New phytologist. - 1979. - 180(2008), 3 vom: 28., Seite 620-630
1. Verfasser: Svennerstam, Henrik (VerfasserIn)
Weitere Verfasser: Ganeteg, Ulrika, Näsholm, Torgny
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2008
Zugriff auf das übergeordnete Werk:The New phytologist
Schlagworte:Journal Article Research Support, Non-U.S. Gov't AAP5 protein, Arabidopsis Amino Acid Transport Systems, Basic Amino Acids Arabidopsis Proteins LHT1 protein, Arabidopsis Nitrates Glutamine 0RH81L854J mehr... Arginine 94ZLA3W45F
LEADER 01000naa a22002652 4500
001 NLM18139104X
003 DE-627
005 20231223161152.0
007 cr uuu---uuuuu
008 231223s2008 xx |||||o 00| ||eng c
024 7 |a 10.1111/j.1469-8137.2008.02589.x  |2 doi 
028 5 2 |a pubmed24n0605.xml 
035 |a (DE-627)NLM18139104X 
035 |a (NLM)18681934 
040 |a DE-627  |b ger  |c DE-627  |e rakwb 
041 |a eng 
100 1 |a Svennerstam, Henrik  |e verfasserin  |4 aut 
245 1 0 |a Root uptake of cationic amino acids by Arabidopsis depends on functional expression of amino acid permease 5 
264 1 |c 2008 
336 |a Text  |b txt  |2 rdacontent 
337 |a ƒaComputermedien  |b c  |2 rdamedia 
338 |a ƒa Online-Ressource  |b cr  |2 rdacarrier 
500 |a Date Completed 03.03.2009 
500 |a Date Revised 16.04.2021 
500 |a published: Print-Electronic 
500 |a Citation Status MEDLINE 
520 |a * Specific transporters mediate uptake of amino acids by plant roots. Earlier studies have indicated that the lysine histidine transporter 1 and amino acid permease 1 participate in this process, but although plant roots have been shown to absorb cationic amino acids with high affinity, neither of these transporters seems to mediate transport of L-arginine (L-Arg) or L-lysine (L-Lys). * Here, a collection of T-DNA knockout mutants were screened for alterations in Arabidopsis root uptake rates of L-Arg and it was found that only the AAP5 mutant displayed clear phenotypic divergence on high concentrations of L-Arg. A second screen using low concentrations of (15)N-labelled L-Arg in the growth media also identified AAP5 as being involved in L-Arg acquisition. * Momentaneous root uptake of basic amino acids was strongly affected in AAP5 mutant lines, but their uptake of other types of amino acids was only marginally affected. Comparisons of the root uptake characteristics of AAP5 and LHT1 mutants corroborated the hypothesis that the two transporters have distinct affinity spectra in planta. * Root uptake of all tested amino acids, except L-aspartic acid (L-Asp), was significantly affected in double AAP5*LHT1 mutants, suggesting that these two transporters account for a major proportion of roots' uptake of amino acids at low concentrations 
650 4 |a Journal Article 
650 4 |a Research Support, Non-U.S. Gov't 
650 7 |a AAP5 protein, Arabidopsis  |2 NLM 
650 7 |a Amino Acid Transport Systems, Basic  |2 NLM 
650 7 |a Amino Acids  |2 NLM 
650 7 |a Arabidopsis Proteins  |2 NLM 
650 7 |a LHT1 protein, Arabidopsis  |2 NLM 
650 7 |a Nitrates  |2 NLM 
650 7 |a Glutamine  |2 NLM 
650 7 |a 0RH81L854J  |2 NLM 
650 7 |a Arginine  |2 NLM 
650 7 |a 94ZLA3W45F  |2 NLM 
700 1 |a Ganeteg, Ulrika  |e verfasserin  |4 aut 
700 1 |a Näsholm, Torgny  |e verfasserin  |4 aut 
773 0 8 |i Enthalten in  |t The New phytologist  |d 1979  |g 180(2008), 3 vom: 28., Seite 620-630  |w (DE-627)NLM09818248X  |x 1469-8137  |7 nnns 
773 1 8 |g volume:180  |g year:2008  |g number:3  |g day:28  |g pages:620-630 
856 4 0 |u http://dx.doi.org/10.1111/j.1469-8137.2008.02589.x  |3 Volltext 
912 |a GBV_USEFLAG_A 
912 |a SYSFLAG_A 
912 |a GBV_NLM 
912 |a GBV_ILN_350 
951 |a AR 
952 |d 180  |j 2008  |e 3  |b 28  |h 620-630