Aldehyde-terminated amorphous carbon substrates for the fabrication of biomolecule arrays
Amorphous carbon thin films are easily deposited at room temperature, readily functionalized with alkene-containing molecules through a UV photochemical reaction, and provide a robust surface capable of supporting chemical and biomolecule array fabrication. Aldehyde-terminated amorphous carbon subst...
| Publié dans: | Langmuir : the ACS journal of surfaces and colloids. - 1985. - 24(2008), 17 vom: 02. Sept., Seite 9198-203 |
|---|---|
| Auteur principal: | |
| Autres auteurs: | , |
| Format: | Article en ligne |
| Langue: | English |
| Publié: |
2008
|
| Accès à la collection: | Langmuir : the ACS journal of surfaces and colloids |
| Sujets: | Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't Aldehydes Amines Oligonucleotides Carbon 7440-44-0 Hydrochloric Acid QTT17582CB |
| Résumé: | Amorphous carbon thin films are easily deposited at room temperature, readily functionalized with alkene-containing molecules through a UV photochemical reaction, and provide a robust surface capable of supporting chemical and biomolecule array fabrication. Aldehyde-terminated amorphous carbon substrates were fabricated via the attachment of a 2-(10-undecen-1-yl)-1,3-dioxolane molecule. The surfaces were then deprotected in 1.5 M HCl to yield an aldehyde-terminated surface that is readily reactive with amine containing molecules. An array of amine-modified oligonucleotides was prepared on aldehyde-terminated surfaces prepared on both amorphous carbon and on gold self-assembled monolayers, and the fluorescence background, feature signal-to-noise ratio, and hybridization densities were compared. The aldehyde-terminated amorphous carbon substrates offer inherently lower background fluorescence intensity and a greater number of hybridization-accessible sites |
|---|---|
| Description: | Date Completed 13.11.2008 Date Revised 20.10.2021 published: Print-Electronic Citation Status MEDLINE |
| ISSN: | 1520-5827 |
| DOI: | 10.1021/la800991t |