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231223s2008 xx |||||o 00| ||eng c |
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|a 10.1021/la800751k
|2 doi
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|e rakwb
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|a eng
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1 |
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|a Rudiuk, Sergii
|e verfasserin
|4 aut
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|a Modulation of photo-oxidative DNA damage by cationic surfactant complexation
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|c 2008
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
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|2 rdamedia
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|a ƒa Online-Ressource
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|a Date Completed 17.09.2008
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|a Date Revised 21.11.2013
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a The natural packaging of DNA in the cell by histones provides a particular environment affecting its sensitivity to oxidative damage. In this work, we used the complexation of DNA by cationic surfactants to modulate the conformation, the dynamics, and the environment of the double helix. Photo-oxidative damage initiated by benzophenone as the photosensitizer on a plasmid DNA complexed by dodecyltrimethylammonium chloride (DTAC), tetradecyltrimethylammonium chloride (TTAC), cetyltrimethyammonium chloride (CTAC) and bromide (CTAB) was detected by agarose gel electrophoresis. By fluorescent titration in the presence of ethidium bromide (EB) and agarose gel electrophoresis, we experimentally confirmed the complexation diagrams with a critical aggregation concentration on DNA matrix (CAC DNA) delimiting two regions of complexation, according to the DNA-phosphate concentration. The study of the photo-oxidative damage shows, for the first time, a direct correlation between the DNA complexation by these surfactants and the efficiency of DNA cleavage, with a maximum corresponding to the CAC DNA for DTAC and CTAC, and to DNA neutralization for CTAC and CTAB. The localization of a photosensitizer having low water solubility, such as benzophenone, inside the hydrophobic domains formed by the surfactant aggregated on DNA, locally increases the photoinduced cleavage by the free radical oxygen species generated. The inefficiency of a water-soluble quencher of hydroxyl radicals, such as mannitol, confirmed this phenomenon. The detection of photo-oxidative damage constitutes a new tool for investigating DNA complexation by cationic surfactants. Moreover, highlighting the drastically increased sensitivity of a complexed DNA to photo-oxidative damage is of crucial importance for the biological use of surfactants as nonviral gene delivery systems
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Cations
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|a Surface-Active Agents
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|a Franceschi-Messant, Sophie
|e verfasserin
|4 aut
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|a Chouini-Lalanne, Nadia
|e verfasserin
|4 aut
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| 700 |
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|a Perez, Emile
|e verfasserin
|4 aut
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|a Rico-Lattes, Isabelle
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Langmuir : the ACS journal of surfaces and colloids
|d 1992
|g 24(2008), 16 vom: 19. Aug., Seite 8452-7
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|x 1520-5827
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|g volume:24
|g year:2008
|g number:16
|g day:19
|g month:08
|g pages:8452-7
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|u http://dx.doi.org/10.1021/la800751k
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