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231223s2008 xx |||||o 00| ||eng c |
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|a 10.1111/j.1469-8137.2008.02546.x
|2 doi
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|a pubmed25n0603.xml
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|a (DE-627)NLM180907948
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|a (NLM)18627491
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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| 100 |
1 |
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|a Forsum, Oskar
|e verfasserin
|4 aut
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| 245 |
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|a Capacities and constraints of amino acid utilization in Arabidopsis
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|c 2008
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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| 338 |
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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| 500 |
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|a Date Completed 18.11.2008
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|a Date Revised 16.04.2021
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|a published: Print
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|a Citation Status MEDLINE
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|a Various amino acids, including both L- and D-enantiomers, may be present in soils, and recent studies have indicated that plants may access such nitrogen (N) forms. Here, the capacity of Arabidopsis to utilize different L- and D-amino acids is investigated and the constraints on this process are explored. Mutants defective in the lysine histidine transporter 1 (LHT1) and transgenic plants overexpressing LHT1 as well as plants expressing D-amino acid-metabolizing enzymes, were used in studies of uptake and growth on various N forms. Arabidopsis absorbed all tested N-forms, but D-enantiomers at lower rates than L-forms. Several L- but no D-forms were effective as N sources. Plants deficient in LHT1 displayed strong growth reductions and plants overexpressing LHT1 showed strong growth enhancement when N was supplied as amino acids, in particular when these were supplied at low concentrations. Several D- amino acids inhibited growth of wild-type plants, while transgenic Arabidopsis-expressing genes encoding D-amino acid-metabolizing enzymes could efficiently utilize such compounds for growth. These results suggest that several amino acids, and in particular L-Gln and L-Asn, promote growth of Arabidopsis, and increased expression of specific amino acid transporters enhances growth on amino acids. The efficiency by which transgenic plants exploit D-amino acids illustrates how plants can be engineered to utilize specific N sources otherwise inaccessible to them
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Amino Acid Transport Systems, Basic
|2 NLM
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| 650 |
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|a Amino Acids
|2 NLM
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| 650 |
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|a Arabidopsis Proteins
|2 NLM
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|a Escherichia coli Proteins
|2 NLM
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| 650 |
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|a Fungal Proteins
|2 NLM
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| 650 |
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|a LHT1 protein, Arabidopsis
|2 NLM
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| 650 |
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|a Nitrogen
|2 NLM
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| 650 |
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|a N762921K75
|2 NLM
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| 700 |
1 |
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|a Svennerstam, Henrik
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Ganeteg, Ulrika
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Näsholm, Torgny
|e verfasserin
|4 aut
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| 773 |
0 |
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|i Enthalten in
|t The New phytologist
|d 1979
|g 179(2008), 4 vom: 15., Seite 1058-1069
|w (DE-627)NLM09818248X
|x 1469-8137
|7 nnas
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| 773 |
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|g volume:179
|g year:2008
|g number:4
|g day:15
|g pages:1058-1069
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|u http://dx.doi.org/10.1111/j.1469-8137.2008.02546.x
|3 Volltext
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