|
|
|
|
| LEADER |
01000naa a22002652 4500 |
| 001 |
NLM180905163 |
| 003 |
DE-627 |
| 005 |
20231223160306.0 |
| 007 |
cr uuu---uuuuu |
| 008 |
231223s2008 xx |||||o 00| ||eng c |
| 024 |
7 |
|
|a 10.1021/la801221r
|2 doi
|
| 028 |
5 |
2 |
|a pubmed24n0603.xml
|
| 035 |
|
|
|a (DE-627)NLM180905163
|
| 035 |
|
|
|a (NLM)18627191
|
| 040 |
|
|
|a DE-627
|b ger
|c DE-627
|e rakwb
|
| 041 |
|
|
|a eng
|
| 100 |
1 |
|
|a Yamazoe, Hironori
|e verfasserin
|4 aut
|
| 245 |
1 |
0 |
|a Facile cell patterning on an albumin-coated surface
|
| 264 |
|
1 |
|c 2008
|
| 336 |
|
|
|a Text
|b txt
|2 rdacontent
|
| 337 |
|
|
|a ƒaComputermedien
|b c
|2 rdamedia
|
| 338 |
|
|
|a ƒa Online-Ressource
|b cr
|2 rdacarrier
|
| 500 |
|
|
|a Date Completed 17.09.2008
|
| 500 |
|
|
|a Date Revised 16.11.2017
|
| 500 |
|
|
|a published: Print-Electronic
|
| 500 |
|
|
|a Citation Status MEDLINE
|
| 520 |
|
|
|a Fabrication of micropatterned surfaces to organize and control cell adhesion and proliferation is an indispensable technique for cell-based technologies. Although several successful strategies for creating cellular micropatterns on substrates have been demonstrated, a complex multistep process and requirements for special and expensive equipment or materials limit their prevalence as a general experimental tool. To circumvent these problems, we describe here a novel facile fabrication method for a micropatterned surface for cell patterning by utilizing the UV-induced conversion of the cell adhesive property of albumin, which is the most abundant protein in blood plasma. An albumin-coated surface was prepared by cross-linking albumin with ethylene glycol diglycidyl ether and subsequent casting of the cross-linked albumin solution on the cell culture dish. While cells did not attach to the albumin surface prepared in this way, UV exposure renders the surface cell-adhesive. Thus, surface micropatterning was achieved simply by exposing the albumin-coated surface to UV light through a mask with the desired pattern. Mouse fibroblast L929 cells were inoculated on the patterned albumin substrates, and cells attached and spread in a highly selective manner according to the UV-irradiated pattern. Although detailed investigation of the molecular-level mechanism concerning the change in cell adhesiveness of the albumin-coated surface is required, the present results would give a novel facile method for the fabrication of cell micropatterned surfaces
|
| 650 |
|
4 |
|a Journal Article
|
| 650 |
|
7 |
|a Water
|2 NLM
|
| 650 |
|
7 |
|a 059QF0KO0R
|2 NLM
|
| 650 |
|
7 |
|a Serum Albumin, Bovine
|2 NLM
|
| 650 |
|
7 |
|a 27432CM55Q
|2 NLM
|
| 700 |
1 |
|
|a Uemura, Toshimasa
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Tanabe, Toshizumi
|e verfasserin
|4 aut
|
| 773 |
0 |
8 |
|i Enthalten in
|t Langmuir : the ACS journal of surfaces and colloids
|d 1992
|g 24(2008), 16 vom: 19. Aug., Seite 8402-4
|w (DE-627)NLM098181009
|x 1520-5827
|7 nnns
|
| 773 |
1 |
8 |
|g volume:24
|g year:2008
|g number:16
|g day:19
|g month:08
|g pages:8402-4
|
| 856 |
4 |
0 |
|u http://dx.doi.org/10.1021/la801221r
|3 Volltext
|
| 912 |
|
|
|a GBV_USEFLAG_A
|
| 912 |
|
|
|a SYSFLAG_A
|
| 912 |
|
|
|a GBV_NLM
|
| 912 |
|
|
|a GBV_ILN_22
|
| 912 |
|
|
|a GBV_ILN_350
|
| 912 |
|
|
|a GBV_ILN_721
|
| 951 |
|
|
|a AR
|
| 952 |
|
|
|d 24
|j 2008
|e 16
|b 19
|c 08
|h 8402-4
|