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231223s2008 xx |||||o 00| ||eng c |
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|a 10.1021/la801327a
|2 doi
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|a pubmed24n0603.xml
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|a eng
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1 |
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|a Iwasaki, Yasuhiko
|e verfasserin
|4 aut
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| 245 |
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|a Site-specific dense immobilization of antibody fragments on polymer brushes supported by silicone nanofilaments
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|c 2008
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
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|2 rdacarrier
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|a Date Completed 17.09.2008
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|a Date Revised 14.08.2008
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a For site-specific dense immobilization of antibodies on a solid support, we prepared phosphorylcholine copolymer brushes on silicone nanofilaments. The nanofilaments were prepared on a silicon wafer by treatment with trichloromethylsilane (MeSiCl 3). To generate Si-OH groups on the nanofilaments, O 2 plasma was irradiated on the surface. Initiators for atom transfer radical polymerization (ATRP) were then coupled on the filaments. Phosphorylcholine copolymer brushes were prepared by a "grafting from" process, and pyridyl disulfide groups were introduced into the polymer chains. F(ab') fragments were then specifically immobilized onto these surfaces via a thiol-disulfide interchange reaction. The amount of antibodies immobilized on the nanofilament-supported copolymer brushes was approximately 65 times greater than that on smooth wafer-supported copolymer brushes
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Antibodies
|2 NLM
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| 650 |
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|a Polymers
|2 NLM
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| 650 |
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|a Silicones
|2 NLM
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| 700 |
1 |
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|a Omichi, Yuki
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Iwata, Ryoko
|e verfasserin
|4 aut
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| 773 |
0 |
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|i Enthalten in
|t Langmuir : the ACS journal of surfaces and colloids
|d 1992
|g 24(2008), 16 vom: 19. Aug., Seite 8427-30
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|g volume:24
|g year:2008
|g number:16
|g day:19
|g month:08
|g pages:8427-30
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|u http://dx.doi.org/10.1021/la801327a
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