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231223s2008 xx |||||o 00| ||eng c |
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|a 10.1080/09593330802028550
|2 doi
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|a pubmed25n0603.xml
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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| 100 |
1 |
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|a Brar, S K
|e verfasserin
|4 aut
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| 245 |
1 |
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|a Bacillus thuringiensis fermentation of wastewater and wastewater sludge--presence and characterization of chitinases
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| 264 |
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|c 2008
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| 336 |
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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| 338 |
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|a ƒa Online-Ressource
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|2 rdacarrier
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|a Date Completed 17.10.2008
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|a Date Revised 24.11.2016
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|a published: Print
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|a Citation Status MEDLINE
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|a This study investigated the presence of chitinases in Bacillus thuringiensis ssp kurstaki HD-1 (Bt) fermented broths of wastewater sludge (non-hydrolyzed and hydrolyzed); starch industry wastewater and soyameal. Chitinase activity was absent in soyameal and present in others. Chitinase demonstrated peaks at pH 4.0 and temperatures 40 and 50 degrees C with higher activity between pH 4-5 and 10-11. The chitinase band on SDS-PAGE was found to be between 36 and 45 kDa for non-hydrolyzed (NH) and hydrolyzed sludge (TH) and starch industry wastewater. The chitinase profile during fermentation showed peaks at 15 and 30 h for non-hydrolyzed and hydrolyzed sludge and 15 and 24 h for starch industry wastewater. Chitinase retained 96-99 % activity after two weeks incubation at room temperature and pH 4. Bioassays with supplementation of Bt chitinases showed 1.2 fold increase in entomotoxicity of wastewater sludge and a small increase in starch industry wastewater. This study sheds light on production of Bt chitinases in alternative media which will have a long term effect on entomotoxicity of these formulations
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Research Support, U.S. Gov't, Non-P.H.S.
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| 650 |
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|a Sewage
|2 NLM
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| 650 |
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|a Water Pollutants, Chemical
|2 NLM
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|a Chitinases
|2 NLM
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| 650 |
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|a EC 3.2.1.14
|2 NLM
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| 700 |
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|a Verma, M
|e verfasserin
|4 aut
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| 700 |
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|a Tyagi, R D
|e verfasserin
|4 aut
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| 700 |
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|a Valéro, J R
|e verfasserin
|4 aut
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| 700 |
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|a Surampalli, R Y
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Environmental technology
|d 1998
|g 29(2008), 2 vom: 04. Feb., Seite 161-70
|w (DE-627)NLM098202545
|x 0959-3330
|7 nnns
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| 773 |
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|g volume:29
|g year:2008
|g number:2
|g day:04
|g month:02
|g pages:161-70
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|u http://dx.doi.org/10.1080/09593330802028550
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