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01000caa a22002652 4500 |
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NLM180754270 |
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DE-627 |
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20250209144501.0 |
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231223s2008 xx ||||| 00| ||chi c |
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|a pubmed25n0603.xml
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|a (DE-627)NLM180754270
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|a (NLM)18611334
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|a DE-627
|b ger
|c DE-627
|e rakwb
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| 041 |
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|a chi
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| 100 |
1 |
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|a Zhang, Xue-mei
|e verfasserin
|4 aut
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| 245 |
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|a The expression and function of pulmonary surfactant protein A in severe acute pancreatitis in rats
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|c 2008
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|a Text
|b txt
|2 rdacontent
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|a ohne Hilfsmittel zu benutzen
|b n
|2 rdamedia
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| 338 |
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|a Band
|b nc
|2 rdacarrier
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| 500 |
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|a Date Completed 04.02.2010
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|a Date Revised 09.07.2008
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|a published: Print
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|a Citation Status MEDLINE
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|a OBJECTIVE: To investigate the expression and function of surfactant protein A (SP-A) in lung in rats with acute lung injury (ALI) induced by severe acute pancreatitis (SAP)
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|a METHODS: Twenty Sprague-Dawley (SD) rats were randomly divided into 2 groups: sham operation group (sham, n=10) and SAP model group (SAP, n=10). SAP model was reproduced in SAP group. In sham group laparotomy only was done. Serum amylase (AMY) levels, partial pressure of oxygen in artery (PaO2) and lung wet/dry (W/D) ratio were determined. SP-A mRNA expression in lung was detected by reverse transcription-polymerase chain reaction (RT-PCR). SP-A protein expression in lung tissues was assessed by immunohistochemistry. The pathologic changes in pancreas and lung were observed 24 hours after reproduction of the model
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|a RESULTS: Compared with sham group, PaO(2) in SAP group was decreased significantly [(96.78+/-3.81) mm Hg vs. (79.24+/-5.84) mm Hg, 1 mm Hg=0.133 kPa, P<0.05]. Serum levels of AMY and W/D ratio in SAP group were remarkably higher than that in sham group [(1 193.41+/-192.54) U/L vs. (7 144.19+/-727.91) U/L, 3.70+/-0.90 vs. 8.57+/-2.45, both P<0.05]. The expression of SP-A mRNA in lung tissues in SAP group was significantly down-regulated compared with that in sham group (1.21+/-0.10 vs. 0.80+/-0.11, P<0.05). The expression of SP-A protein in lung in SAP group was significantly decreased than that in sham group (7 982.22+/-3 689.57 vs. 3 497.99+/-2 958.21, P<0.05). The pathologic changes in pancreas and lung were marked in SAP group. The expression of SP-A protein and mRNA showed significant negative correlation with the extent of lung injury (r1= -0.876, P<0.01; r2= -0.713, P<0.05)
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|a CONCLUSION: SP-A decreases remarkably in ALI induced by SAP. Type II alveolar epithelial cells might play an important role in ALI induced by SAP
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|a English Abstract
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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| 650 |
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|a Pulmonary Surfactant-Associated Protein A
|2 NLM
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| 700 |
1 |
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|a Chen, Hai-long
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Wang, Zhao-hui
|e verfasserin
|4 aut
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| 773 |
0 |
8 |
|i Enthalten in
|t Zhongguo wei zhong bing ji jiu yi xue = Chinese critical care medicine = Zhongguo weizhongbing jijiuyixue
|d 1998
|g 20(2008), 7 vom: 16. Juli, Seite 390-2
|w (DE-627)NLM098227793
|x 1003-0603
|7 nnns
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| 773 |
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|g volume:20
|g year:2008
|g number:7
|g day:16
|g month:07
|g pages:390-2
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|a GBV_ILN_350
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| 951 |
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|a AR
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| 952 |
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|d 20
|j 2008
|e 7
|b 16
|c 07
|h 390-2
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