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231223s2008 xx |||||o 00| ||eng c |
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|a 10.1021/la800643e
|2 doi
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|a pubmed24n0601.xml
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|a eng
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1 |
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|a Fukuba, Shin-Ya
|e verfasserin
|4 aut
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1 |
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|a Nonlinear optical detection of proteins based on localized surface plasmons in surface immobilized gold nanospheres
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|c 2008
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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| 338 |
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|a ƒa Online-Ressource
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|2 rdacarrier
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| 500 |
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|a Date Completed 08.09.2008
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|a Date Revised 30.07.2008
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a A new nonlinear optical method is presented to detect proteins binding to a gold surface without using fluorescent-dye labeling. After exposure of the protein-binding surface to a gold nanosphere solution, the nanospheres are immobilized above a gold surface with a nanogap supported by the protein. The gold nanospheres immobilized on the gold surface show strong localized surface plasmon (LSP) resonance, and the formation of this structure results in a marked increase in the optical second harmonic (SH) activity of the gold surface arising from a large enhancement of the electric field localized adjacent to the nanospheres on the LSP resonance. The SH image, therefore, gives a high contrast ratio, 7.0:1, of protein-binding spots to control spots. The contrast ratio is much greater than those obtained by linear reflectivity imaging
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|a Journal Article
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|a Proteins
|2 NLM
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|a Gold
|2 NLM
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| 650 |
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|a 7440-57-5
|2 NLM
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| 700 |
1 |
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|a Tsuboi, Kazuma
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Abe, Shinya
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Kajikawa, Kotaro
|e verfasserin
|4 aut
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| 773 |
0 |
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|i Enthalten in
|t Langmuir : the ACS journal of surfaces and colloids
|d 1992
|g 24(2008), 15 vom: 05. Aug., Seite 8367-72
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|x 1520-5827
|7 nnns
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|g volume:24
|g year:2008
|g number:15
|g day:05
|g month:08
|g pages:8367-72
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|u http://dx.doi.org/10.1021/la800643e
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