Effect of temperature on n-tetradecane emulsion in the presence of phospholipid DPPC and enzyme lipase or phospholipase A2

Zeta potentials and effective diameters of n-tetradecane emulsions in 1 M ethanol were investigated in the presence of 1,2-dipalmitoyl- sn-glycero-3-phosphocholine (DPPC) (1 mg/100 mL), Candida cylindracea lipase (CCL), and phospholipase PLA2 (1 mg/100 mL) at 20, 37, and 45 degrees C. The enzyme was...

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Veröffentlicht in:Langmuir : the ACS journal of surfaces and colloids. - 1991. - 24(2008), 14 vom: 15. Juli, Seite 7413-20
1. Verfasser: Wiacek, Agnieszka Ewa (VerfasserIn)
Weitere Verfasser: Holysz, Lucyna, Chibowski, Emil
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2008
Zugriff auf das übergeordnete Werk:Langmuir : the ACS journal of surfaces and colloids
Schlagworte:Journal Article Research Support, Non-U.S. Gov't Alkanes Emulsions Solutions n-tetradecane 03LY784Y58 1,2-Dipalmitoylphosphatidylcholine 2644-64-6 Ethanol mehr... 3K9958V90M Lipase EC 3.1.1.3 Phospholipases A2 EC 3.1.1.4
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245 1 0 |a Effect of temperature on n-tetradecane emulsion in the presence of phospholipid DPPC and enzyme lipase or phospholipase A2 
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520 |a Zeta potentials and effective diameters of n-tetradecane emulsions in 1 M ethanol were investigated in the presence of 1,2-dipalmitoyl- sn-glycero-3-phosphocholine (DPPC) (1 mg/100 mL), Candida cylindracea lipase (CCL), and phospholipase PLA2 (1 mg/100 mL) at 20, 37, and 45 degrees C. The enzyme was added at the beginning of mechanical emulsion homogenization or 1 min before the end of stirring for 10 min at 10,000 rpm. It was found that DPPC decreases the negative zeta potentials at all three temperatures. The decrease was largest at 20 degrees C and smallest at 45 degrees C. The influence of the enzymes on the zeta potentials depended on the enzyme kind, time of its injection, and temperature. More negative values of the zeta potentials relative to n-C14H30/DPPC droplets were obtained if the lipase was present. Generally, the effective diameters correlate with the zeta potentials, i.e., lower zeta potential corresponds with bigger effective diameter. Possible reasons for the observed changes of the measured parameters are discussed 
650 4 |a Journal Article 
650 4 |a Research Support, Non-U.S. Gov't 
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650 7 |a n-tetradecane  |2 NLM 
650 7 |a 03LY784Y58  |2 NLM 
650 7 |a 1,2-Dipalmitoylphosphatidylcholine  |2 NLM 
650 7 |a 2644-64-6  |2 NLM 
650 7 |a Ethanol  |2 NLM 
650 7 |a 3K9958V90M  |2 NLM 
650 7 |a Lipase  |2 NLM 
650 7 |a EC 3.1.1.3  |2 NLM 
650 7 |a Phospholipases A2  |2 NLM 
650 7 |a EC 3.1.1.4  |2 NLM 
700 1 |a Holysz, Lucyna  |e verfasserin  |4 aut 
700 1 |a Chibowski, Emil  |e verfasserin  |4 aut 
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