The Lmgpi15 gene, encoding a component of the glycosylphosphatidylinositol anchor biosynthesis pathway, is required for morphogenesis and pathogenicity in Leptosphaeria maculans

Random insertional mutagenesis was used to investigate pathogenicity determinants in Leptosphaeria maculans. One tagged nonpathogenic mutant, termed m20, was analysed in detail here. The mutant phenotype was investigated by microscopic analyses of infected plant tissues and in vitro growth assays. C...

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Veröffentlicht in:The New phytologist. - 1979. - 179(2008), 4 vom: 01., Seite 1105-1120
1. Verfasser: Remy, Estelle (VerfasserIn)
Weitere Verfasser: Meyer, Michel, Blaise, Françoise, Simon, Uwe K, Kuhn, Diana, Chabirand, Mélanie, Riquelme, Meritxell, Balesdent, Marie-Hélène, Rouxel, Thierry
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2008
Zugriff auf das übergeordnete Werk:The New phytologist
Schlagworte:Journal Article Research Support, Non-U.S. Gov't Fungal Proteins Glycosylphosphatidylinositols Gpi15 protein, S cerevisiae Membrane Proteins Recombinant Fusion Proteins Saccharomyces cerevisiae Proteins Green Fluorescent Proteins 147336-22-9
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245 1 4 |a The Lmgpi15 gene, encoding a component of the glycosylphosphatidylinositol anchor biosynthesis pathway, is required for morphogenesis and pathogenicity in Leptosphaeria maculans 
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500 |a Date Revised 16.04.2021 
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500 |a Citation Status MEDLINE 
520 |a Random insertional mutagenesis was used to investigate pathogenicity determinants in Leptosphaeria maculans. One tagged nonpathogenic mutant, termed m20, was analysed in detail here. The mutant phenotype was investigated by microscopic analyses of infected plant tissues and in vitro growth assays. Complementation and silencing experiments were used to identify the altered gene. Its function was determined by bioinformatics analyses, cell biology experiments and functional studies. The mutant was blocked at the invasive growth phase after an unaffected initial penetration stage, and displayed a reduced growth rate and an aberrant hyphal morphology in vitro. The T-DNA insertion occurred in the intergenic region between two head-to-tail genes, leading to a complex deregulation of their expression. The unique gene accounting for the mutant phenotype was suggested to be the orthologue of the poorly conserved Saccharomyces cerevisiae gpi15, which encodes for one component of the glycosylphosphatidylinositol (GPI) anchor biosynthesis pathway. Consistent with this predicted function, a functional translational fusion with the green fluorescent protein (GFP) was targeted to the endoplasmic reticulum. Moreover, the mutant exhibited an altered cell wall and addition of glucosamine relieved growth defects. It is concluded that the GPI anchor biosynthetic pathway is required for morphogenesis, cell wall integrity and pathogenicity in Leptosphaeria maculans 
650 4 |a Journal Article 
650 4 |a Research Support, Non-U.S. Gov't 
650 7 |a Fungal Proteins  |2 NLM 
650 7 |a Glycosylphosphatidylinositols  |2 NLM 
650 7 |a Gpi15 protein, S cerevisiae  |2 NLM 
650 7 |a Membrane Proteins  |2 NLM 
650 7 |a Recombinant Fusion Proteins  |2 NLM 
650 7 |a Saccharomyces cerevisiae Proteins  |2 NLM 
650 7 |a Green Fluorescent Proteins  |2 NLM 
650 7 |a 147336-22-9  |2 NLM 
700 1 |a Meyer, Michel  |e verfasserin  |4 aut 
700 1 |a Blaise, Françoise  |e verfasserin  |4 aut 
700 1 |a Simon, Uwe K  |e verfasserin  |4 aut 
700 1 |a Kuhn, Diana  |e verfasserin  |4 aut 
700 1 |a Chabirand, Mélanie  |e verfasserin  |4 aut 
700 1 |a Riquelme, Meritxell  |e verfasserin  |4 aut 
700 1 |a Balesdent, Marie-Hélène  |e verfasserin  |4 aut 
700 1 |a Rouxel, Thierry  |e verfasserin  |4 aut 
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