Expression and activity of sulfate transporters and APS reductase in curly kale in response to sulfate deprivation and re-supply

Both activity and expression of sulfate transporters and APS reductase in plants are modulated by the sulfur status of the plant. To examine the regulatory mechanisms in curly kale (Brassica oleracea L.), the sulfate supply was manipulated by the transfer of seedlings to sulfate-deprived conditions,...

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Publié dans:Journal of plant physiology. - 1979. - 166(2009), 2 vom: 30. Jan., Seite 168-79
Auteur principal: Koralewska, Aleksandra (Auteur)
Autres auteurs: Buchner, Peter, Stuiver, C Elisabeth E, Posthumus, Freek S, Kopriva, Stanislav, Hawkesford, Malcolm J, De Kok, Luit J
Format: Article en ligne
Langue:English
Publié: 2009
Accès à la collection:Journal of plant physiology
Sujets:Journal Article Research Support, Non-U.S. Gov't Membrane Transport Proteins Nitrates Plant Proteins RNA, Messenger Sulfates Sulfhydryl Compounds Oxidoreductases Acting on Sulfur Group Donors EC 1.8.- plus... adenylylsulfate reductase EC 1.8.99.2
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520 |a Both activity and expression of sulfate transporters and APS reductase in plants are modulated by the sulfur status of the plant. To examine the regulatory mechanisms in curly kale (Brassica oleracea L.), the sulfate supply was manipulated by the transfer of seedlings to sulfate-deprived conditions, which resulted in an up to 3-fold increase in the sulfate uptake capacity by the root, accompanied by an induction of transcript abundances of the Group 1 and 4 sulfate transporters in root and shoot. Upon sulfate re-supply, there was no correlation between the activity and expression of the sulfate transporters. Despite the decrease in the abundance of the sulfate transporter transcripts, especially at the onset of the sulfate re-supply, the sulfate uptake capacity was affected very little for up to 96h. There was no relationship between changes in the sulfate or thiol content and activity and expression of the sulfate transporters. Thus, their significance as regulatory signal compounds remains unresolved. The activity and expression of APS reductase, which was enhanced strongly only in the shoots of sulfate-deprived plants, and rapidly decreased again upon sulfate re-supply, corresponded with changes in thiol content, consistent with this pool having a role as a regulatory signal 
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650 7 |a Oxidoreductases Acting on Sulfur Group Donors  |2 NLM 
650 7 |a EC 1.8.-  |2 NLM 
650 7 |a adenylylsulfate reductase  |2 NLM 
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700 1 |a Buchner, Peter  |e verfasserin  |4 aut 
700 1 |a Stuiver, C Elisabeth E  |e verfasserin  |4 aut 
700 1 |a Posthumus, Freek S  |e verfasserin  |4 aut 
700 1 |a Kopriva, Stanislav  |e verfasserin  |4 aut 
700 1 |a Hawkesford, Malcolm J  |e verfasserin  |4 aut 
700 1 |a De Kok, Luit J  |e verfasserin  |4 aut 
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