Element-specific microtomographic imaging of Drosophila brain stained with high-Z probes

An application of X-ray microtomography to the Drosophila adult brain stained with colloidal gold and a platinum compound is described. The transparency of biological tissue to hard X-rays enables tomographic visualization of the three-dimensional structure of tissue entrails. Each high-Z element wa...

Ausführliche Beschreibung

Bibliographische Detailangaben
Veröffentlicht in:Journal of synchrotron radiation. - 1994. - 15(2008), Pt 4 vom: 07. Juli, Seite 374-7
1. Verfasser: Mizutani, Ryuta (VerfasserIn)
Weitere Verfasser: Takeuchi, Akihisa, Akamatsu, Genta, Uesugi, Kentaro, Suzuki, Yoshio
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2008
Zugriff auf das übergeordnete Werk:Journal of synchrotron radiation
Schlagworte:Journal Article
LEADER 01000naa a22002652 4500
001 NLM180200321
003 DE-627
005 20231223155055.0
007 cr uuu---uuuuu
008 231223s2008 xx |||||o 00| ||eng c
024 7 |a 10.1107/S0909049508003725  |2 doi 
028 5 2 |a pubmed24n0601.xml 
035 |a (DE-627)NLM180200321 
035 |a (NLM)18552430 
040 |a DE-627  |b ger  |c DE-627  |e rakwb 
041 |a eng 
100 1 |a Mizutani, Ryuta  |e verfasserin  |4 aut 
245 1 0 |a Element-specific microtomographic imaging of Drosophila brain stained with high-Z probes 
264 1 |c 2008 
336 |a Text  |b txt  |2 rdacontent 
337 |a ƒaComputermedien  |b c  |2 rdamedia 
338 |a ƒa Online-Ressource  |b cr  |2 rdacarrier 
500 |a Date Completed 25.08.2008 
500 |a Date Revised 16.06.2008 
500 |a published: Print-Electronic 
500 |a Citation Status MEDLINE 
520 |a An application of X-ray microtomography to the Drosophila adult brain stained with colloidal gold and a platinum compound is described. The transparency of biological tissue to hard X-rays enables tomographic visualization of the three-dimensional structure of tissue entrails. Each high-Z element was visualized as a three-dimensional structure from the difference absorption coefficient image at the corresponding L(III) absorption edge. The cortex of the optic lobe was selectively visualized by the specific adsorption of colloidal gold. The entire structure revealed by the platinum impregnation allowed the anatomical assignment of the gold-stained structures. Selective staining and specific visualization of biological tissues at micrometer resolution should elucidate the three-dimensional cellular organization essential for the understanding and application of biological microstructures 
650 4 |a Journal Article 
700 1 |a Takeuchi, Akihisa  |e verfasserin  |4 aut 
700 1 |a Akamatsu, Genta  |e verfasserin  |4 aut 
700 1 |a Uesugi, Kentaro  |e verfasserin  |4 aut 
700 1 |a Suzuki, Yoshio  |e verfasserin  |4 aut 
773 0 8 |i Enthalten in  |t Journal of synchrotron radiation  |d 1994  |g 15(2008), Pt 4 vom: 07. Juli, Seite 374-7  |w (DE-627)NLM09824129X  |x 1600-5775  |7 nnns 
773 1 8 |g volume:15  |g year:2008  |g number:Pt 4  |g day:07  |g month:07  |g pages:374-7 
856 4 0 |u http://dx.doi.org/10.1107/S0909049508003725  |3 Volltext 
912 |a GBV_USEFLAG_A 
912 |a SYSFLAG_A 
912 |a GBV_NLM 
912 |a GBV_ILN_40 
912 |a GBV_ILN_350 
912 |a GBV_ILN_2005 
951 |a AR 
952 |d 15  |j 2008  |e Pt 4  |b 07  |c 07  |h 374-7