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231223s2008 xx |||||o 00| ||eng c |
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|a 10.1093/jxb/ern149
|2 doi
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|a pubmed24n1332.xml
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|a (DE-627)NLM180199544
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|a (NLM)18552352
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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| 100 |
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|a Malik, Meghna R
|e verfasserin
|4 aut
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| 245 |
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|a Isolation of an embryogenic line from non-embryogenic Brassica napus cv. Westar through microspore embryogenesis
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|c 2008
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Completed 28.08.2008
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|a Date Revised 16.03.2024
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a Brassica napus cultivar Westar is non-embryogenic under all standard protocols for induction of microspore embryogenesis; however, the rare embryos produced in Westar microspore cultures, induced with added brassinosteroids, were found to develop into heritably stable embryogenic lines after chromosome doubling. One of the Westar-derived doubled haploid (DH) lines, DH-2, produced up to 30% the number of embryos as the highly embryogenic B. napus line, Topas DH4079. Expression analysis of marker genes for embryogenesis in Westar and the derived DH-2 line, using real-time reverse transcription-PCR, revealed that the timely expression of embryogenesis-related genes such as LEAFY COTYLEDON1 (LEC1), LEC2, ABSCISIC ACID INSENSITIVE3, and BABY BOOM1, and an accompanying down-regulation of pollen-related transcripts, were associated with commitment to embryo development in Brassica microspores. Microarray comparisons of 7 d cultures of Westar and Westar DH-2, using a B. napus seed-focused cDNA array (10 642 unigenes), identified highly expressed genes related to protein synthesis, translation, and response to stimulus (Gene Ontology) in the embryogenic DH-2 microspore-derived cell cultures. In contrast, transcripts for pollen-expressed genes were predominant in the recalcitrant Westar microspores. Besides being embryogenic, DH-2 plants showed alterations in morphology and architecture as compared with Westar, for example epinastic leaves, non-abscised petals, pale flower colour, and longer lateral branches. Auxin, cytokinin, and abscisic acid (ABA) profiles in young leaves, mature leaves, and inflorescences of Westar and DH-2 revealed no significant differences that could account for the alterations in embryogenic potential or phenotype. Various mechanisms accounting for the increased capacity for embryogenesis in Westar-derived DH lines are considered
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|a Comparative Study
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Brassica napus
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|a embryogenesis
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|a microarray
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| 650 |
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|a microspore
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| 650 |
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|a transcript profiling
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| 650 |
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|a Plant Growth Regulators
|2 NLM
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| 650 |
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|a Plant Proteins
|2 NLM
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| 700 |
1 |
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|a Wang, Feng
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Dirpaul, Joan M
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Zhou, Ning
|e verfasserin
|4 aut
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| 700 |
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|a Hammerlindl, Joe
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Keller, Wilf
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Abrams, Suzanne R
|e verfasserin
|4 aut
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| 700 |
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|a Ferrie, Alison M R
|e verfasserin
|4 aut
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| 700 |
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|a Krochko, Joan E
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Journal of experimental botany
|d 1985
|g 59(2008), 10 vom: 12., Seite 2857-73
|w (DE-627)NLM098182706
|x 1460-2431
|7 nnns
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| 773 |
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|g volume:59
|g year:2008
|g number:10
|g day:12
|g pages:2857-73
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| 856 |
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|u http://dx.doi.org/10.1093/jxb/ern149
|3 Volltext
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|d 59
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