Detection of S-phase cell cycle progression using 5-ethynyl-2'-deoxyuridine incorporation with click chemistry, an alternative to using 5-bromo-2'-deoxyuridine antibodies
The 5-bromo-2'-deoxyuridine (BrdU) labeling of cells followed by antibody staining has been the standard method for direct measurement of cells in the S-phase. Described is an improved method for the detection of S-phase cell cycle progression based upon the application of click chemistry, the...
Ausführliche Beschreibung
Bibliographische Detailangaben
| Veröffentlicht in: | BioTechniques. - 1988. - 44(2008), 7 vom: 15. Juni, Seite 927-9
|
|---|
| 1. Verfasser: |
Buck, Suzanne B
(VerfasserIn) |
|---|
| Weitere Verfasser: |
Bradford, Jolene,
Gee, Kyle R,
Agnew, Brian J,
Clarke, Scott T,
Salic, Adrian |
|---|
| Format: | Online-Aufsatz
|
|---|
| Sprache: | English |
|---|
| Veröffentlicht: |
2008
|
|---|
| Zugriff auf das übergeordnete Werk: | BioTechniques
|
|---|
| Schlagworte: | Journal Article
Antibodies
DNA
9007-49-2
Bromodeoxyuridine
G34N38R2N1
5-ethynyl-2'-deoxyuridine
G373S00W2J
Deoxyuridine
W78I7AY22C |
|---|