Over-expression of a zeatin O-glucosylation gene in maize leads to growth retardation and tasselseed formation

To study the effects of cytokinin O-glucosylation in monocots, maize (Zea mays L.) transformants harbouring the ZOG1 gene (encoding a zeatin O-glucosyltransferase from Phaseolus lunatus L.) under the control of the constitutive ubiquitin (Ubi) promoter were generated. The roots and leaves of the tra...

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Bibliographische Detailangaben
Veröffentlicht in:	Journal of experimental botany. - 1985. - 59(2008), 10 vom: 15., Seite 2673-86
1. Verfasser:	Pineda Rodo, Albert (VerfasserIn)
Weitere Verfasser:	Brugière, Norbert, Vankova, Radomira, Malbeck, Jiri, Olson, Jaleh M, Haines, Sara C, Martin, Ruth C, Habben, Jeffrey E, Mok, David W S, Mok, Machteld C
Format:	Online-Aufsatz
Sprache:	English
Veröffentlicht:	2008
Zugriff auf das übergeordnete Werk:	Journal of experimental botany
Schlagworte:	Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Corn Zea mays cytokinin plant development tasselseed zeatin O-glucosyltransferase mehr... Glucosides Plant Proteins zeatin-O-glucoside Chlorophyll 1406-65-1 Zeatin 7I6OOJ9GR6 Glucosyltransferases EC 2.4.1.- UDPglucose zeatin O-glucosyltransferase, plant EC 2.4.1.203

Beschreibung
Zusammenfassung:	To study the effects of cytokinin O-glucosylation in monocots, maize (Zea mays L.) transformants harbouring the ZOG1 gene (encoding a zeatin O-glucosyltransferase from Phaseolus lunatus L.) under the control of the constitutive ubiquitin (Ubi) promoter were generated. The roots and leaves of the transformants had greatly increased levels of zeatin-O-glucoside. The vegetative characteristics of hemizygous and homozygous Ubi:ZOG1 plants resembled those of cytokinin-deficient plants, including shorter stature, thinner stems, narrower leaves, smaller meristems, and increased root mass and branching. Transformant leaves had a higher chlorophyll content and increased levels of active cytokinins compared with those of non-transformed sibs. The Ubi:ZOG1 plants exhibited delayed senescence when grown in the spring/summer. While hemizygous transformants had reduced tassels with fewer spikelets and normal viable pollen, homozygotes had very small tassels and feminized tassel florets, resembling tasselseed phenotypes. Such modifications of the reproductive phase were unexpected and demonstrate a link between cytokinins and sex-specific floral development in monocots
Beschreibung:	Date Completed 28.08.2008 Date Revised 10.03.2022 published: Print-Electronic Citation Status MEDLINE
ISSN:	1460-2431
DOI:	10.1093/jxb/ern137