|
|
|
|
| LEADER |
01000caa a22002652 4500 |
| 001 |
NLM179911708 |
| 003 |
DE-627 |
| 005 |
20231227123918.0 |
| 007 |
cr uuu---uuuuu |
| 008 |
231223s2008 xx |||||o 00| ||eng c |
| 024 |
7 |
|
|a 10.1093/jxb/ern141
|2 doi
|
| 028 |
5 |
2 |
|a pubmed24n1222.xml
|
| 035 |
|
|
|a (DE-627)NLM179911708
|
| 035 |
|
|
|a (NLM)18515824
|
| 040 |
|
|
|a DE-627
|b ger
|c DE-627
|e rakwb
|
| 041 |
|
|
|a eng
|
| 100 |
1 |
|
|a Zhou, Yu
|e verfasserin
|4 aut
|
| 245 |
1 |
0 |
|a Influence of viral genes on the cell-to-cell spread of RNA silencing
|
| 264 |
|
1 |
|c 2008
|
| 336 |
|
|
|a Text
|b txt
|2 rdacontent
|
| 337 |
|
|
|a ƒaComputermedien
|b c
|2 rdamedia
|
| 338 |
|
|
|a ƒa Online-Ressource
|b cr
|2 rdacarrier
|
| 500 |
|
|
|a Date Completed 28.08.2008
|
| 500 |
|
|
|a Date Revised 13.12.2023
|
| 500 |
|
|
|a published: Print-Electronic
|
| 500 |
|
|
|a Citation Status MEDLINE
|
| 520 |
|
|
|a The turnip crinkle virus-based vector TCV-GFP Delta CP had been devised previously to study cell-to-cell and long-distance spread of virus-induced RNA silencing. TCV-GFP Delta CP, which had been constructed by replacing the coat protein (CP) gene with a green fluorescent protein (GFP) coding sequence, was able to induce RNA silencing in single epidermal cells, from which RNA silencing spread from cell-to-cell. Using this unique local silencing assay together with mutagenesis analysis, two TCV genes, p8 and p9, which were involved in the intercellular spread of virus-induced RNA silencing, were identified. TCV-GFP Delta CP and its p8- or p9-mutated derivatives, TCVmp8-GFP Delta CP and TCVmp9-GFP Delta CP, replicated efficiently but were restricted to single Nicotiana benthamiana epidermal cells. TCV-GFP Delta CP, TCVmp8-GFP Delta CP, or TCVmp9-GFP Delta CP was able to initiate RNA silencing that targeted and degraded recombinant viral RNAs in inoculated leaves of the GFP-expressing N. benthamiana line 16c. However, cell-to-cell spread of silencing to form silencing foci was triggered only by TCV-GFP Delta CP. Non-replicating TCVmp88-GFP Delta CP and TCVmp28mp88-GFP Delta CP with dysfunctional replicase genes, and single-stranded gfp RNA did not induce RNA silencing. Transient expression of the TCV p9 protein could effectively complement TCVmp9-GFP Delta CP to facilitate intercellular spread of silencing. These data suggest that the plant cellular trafficking machinery could hijack functional viral proteins to permit cell-to-cell movement of RNA silencing
|
| 650 |
|
4 |
|a Journal Article
|
| 650 |
|
4 |
|a Research Support, Non-U.S. Gov't
|
| 650 |
|
4 |
|a Cell-to-cell movement
|
| 650 |
|
4 |
|a Nicotiana benthamiana
|
| 650 |
|
4 |
|a RNA silencing
|
| 650 |
|
4 |
|a TCV
|
| 650 |
|
4 |
|a viral movement proteins
|
| 650 |
|
7 |
|a Viral Proteins
|2 NLM
|
| 650 |
|
7 |
|a Green Fluorescent Proteins
|2 NLM
|
| 650 |
|
7 |
|a 147336-22-9
|2 NLM
|
| 700 |
1 |
|
|a Ryabov, Eugene
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Zhang, Xuemei
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Hong, Yiguo
|e verfasserin
|4 aut
|
| 773 |
0 |
8 |
|i Enthalten in
|t Journal of experimental botany
|d 1985
|g 59(2008), 10 vom: 08., Seite 2803-13
|w (DE-627)NLM098182706
|x 1460-2431
|7 nnns
|
| 773 |
1 |
8 |
|g volume:59
|g year:2008
|g number:10
|g day:08
|g pages:2803-13
|
| 856 |
4 |
0 |
|u http://dx.doi.org/10.1093/jxb/ern141
|3 Volltext
|
| 912 |
|
|
|a GBV_USEFLAG_A
|
| 912 |
|
|
|a SYSFLAG_A
|
| 912 |
|
|
|a GBV_NLM
|
| 912 |
|
|
|a GBV_ILN_350
|
| 951 |
|
|
|a AR
|
| 952 |
|
|
|d 59
|j 2008
|e 10
|b 08
|h 2803-13
|