A quantitative real-time PCR method for absolute telomere length

Telomere shortening is an important risk factor for cancer and accelerated aging. Here we describe the development of a simple and reproducible method to measure absolute telomere length. Based on Cawthon's quantitative real-time PCR (qRT-PCR) assay, our method uses an oligomer standard that ca...

Description complète

Détails bibliographiques
Publié dans:BioTechniques. - 1993. - 44(2008), 6 vom: 01. Mai, Seite 807-9
Auteur principal: O'Callaghan, Nathan (Auteur)
Autres auteurs: Dhillon, Varinderpal, Thomas, Philip, Fenech, Michael
Format: Article en ligne
Langue:English
Publié: 2008
Accès à la collection:BioTechniques
Sujets:Journal Article Research Support, Non-U.S. Gov't