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|a eng
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| 100 |
1 |
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|a O'Callaghan, Nathan
|e verfasserin
|4 aut
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| 245 |
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|a A quantitative real-time PCR method for absolute telomere length
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|c 2008
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
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|a Date Completed 04.08.2008
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|a Date Revised 09.04.2022
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|a published: Print
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|a Citation Status MEDLINE
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| 520 |
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|a Telomere shortening is an important risk factor for cancer and accelerated aging. Here we describe the development of a simple and reproducible method to measure absolute telomere length. Based on Cawthon's quantitative real-time PCR (qRT-PCR) assay, our method uses an oligomer standard that can be used to generate absolute telomere length values rather than relative quantification. We demonstrate a strong correlation between this improved method and the "gold standard" of telomere length measurement-terminal restriction fragment analysis (TRF) by Southern hybridization. The capability to generate absolute telomere length values should allow a more direct comparison of results between experiments within and between laboratories
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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| 700 |
1 |
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|a Dhillon, Varinderpal
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Thomas, Philip
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Fenech, Michael
|e verfasserin
|4 aut
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| 773 |
0 |
8 |
|i Enthalten in
|t BioTechniques
|d 1993
|g 44(2008), 6 vom: 01. Mai, Seite 807-9
|w (DE-627)NLM012627046
|x 0736-6205
|7 nnns
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| 773 |
1 |
8 |
|g volume:44
|g year:2008
|g number:6
|g day:01
|g month:05
|g pages:807-9
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|u http://dx.doi.org/10.2144/000112761
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