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231223s2008 xx |||||o 00| ||eng c |
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|a 10.1021/la703984z
|2 doi
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|a pubmed24n0598.xml
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|a (DE-627)NLM17937821X
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|a (NLM)18459755
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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1 |
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|a Hoeben, Freek J M
|e verfasserin
|4 aut
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| 245 |
1 |
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|a Polymyxin-coated Au and carbon nanotube electrodes for stable [NiFe]-hydrogenase film voltammetry
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|c 2008
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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| 338 |
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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| 500 |
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|a Date Completed 22.07.2008
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|a Date Revised 19.11.2009
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a We report on the use of polymyxin (PM), a cyclic cationic lipodecapeptide, as an electrode modifier for studying protein film voltammetry (PFV) on Au and single-walled carbon nanotube (SWNT) electrodes. Pretreating the electrodes with PM allows for the subsequent immobilization of an active submonolayer of [NiFe]-hydrogenase from Allochromatium vinosum ( Av H2ase). Probed by cyclic voltammetry (CV), the adsorbed enzyme exhibits characteristic electrocatalytic behavior that is stable for several hours under continuous potential cycling. An unexpected feature of the immobilization procedure is that the presence of chloride ions is a prerequisite for obtaining electrocatalytic activity. Atomic force microscopy (AFM) relates the observed catalytic activity to enzymatic adsorption at the PM/Au(111) surface, and a combination of concentration-dependent CV and AFM is used to investigate the interaction between the enzyme and the PM layer
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Bacterial Proteins
|2 NLM
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|a Chlorides
|2 NLM
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|a Enzymes, Immobilized
|2 NLM
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|a Nanotubes, Carbon
|2 NLM
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|a Polymyxins
|2 NLM
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|a Gold
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|a 7440-57-5
|2 NLM
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|a Hydrogenase
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|2 NLM
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|a Heller, Iddo
|e verfasserin
|4 aut
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|a Albracht, Simon P J
|e verfasserin
|4 aut
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|a Dekker, Cees
|e verfasserin
|4 aut
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|a Lemay, Serge G
|e verfasserin
|4 aut
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|a Heering, Hendrik A
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Langmuir : the ACS journal of surfaces and colloids
|d 1992
|g 24(2008), 11 vom: 03. Juni, Seite 5925-31
|w (DE-627)NLM098181009
|x 1520-5827
|7 nnns
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| 773 |
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|g volume:24
|g year:2008
|g number:11
|g day:03
|g month:06
|g pages:5925-31
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|u http://dx.doi.org/10.1021/la703984z
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