Root tip-dependent, active riboflavin secretion by Hyoscyamus albus hairy roots under iron deficiency

Hyoscyamus albus hairy roots with/without an exogenous gene (11 clones) were established by inoculation of Agrobacterium rhizogenes. All clones cultured under iron-deficient condition secreted riboflavin from the root tips into the culture medium and the productivity depended on the number and size...

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Veröffentlicht in:Plant physiology and biochemistry : PPB. - 1991. - 46(2008), 4 vom: 15. Apr., Seite 452-60
1. Verfasser: Higa, Ataru (VerfasserIn)
Weitere Verfasser: Miyamoto, Erika, ur Rahman, Laiq, Kitamura, Yoshie
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2008
Zugriff auf das übergeordnete Werk:Plant physiology and biochemistry : PPB
Schlagworte:Journal Article Research Support, Non-U.S. Gov't Riboflavin TLM2976OFR
Beschreibung
Zusammenfassung:Hyoscyamus albus hairy roots with/without an exogenous gene (11 clones) were established by inoculation of Agrobacterium rhizogenes. All clones cultured under iron-deficient condition secreted riboflavin from the root tips into the culture medium and the productivity depended on the number and size of root tips among the clones. A decline of pH was observed before riboflavin production and root development. By studying effects of proton-pump inhibitors, medium acidification with external organic acid, and riboflavin addition upon pH change and riboflavin productivity, we indicate that riboflavin efflux is not directly connected to active pH reduction, and more significantly active riboflavin secretion occurs as a response to an internal requirement in H. albus hairy roots under iron deficiency
Beschreibung:Date Completed 22.07.2008
Date Revised 03.12.2021
published: Print-Electronic
Citation Status MEDLINE
ISSN:1873-2690
DOI:10.1016/j.plaphy.2008.01.004