Fast mixing and reaction initiation control of single-enzyme kinetics in confined volumes
A device with femtoliter-scale chambers and controlled reaction initiation was developed for single-molecule enzymology. Initially separated substrate and enzyme streams were rapidly mixed in a microfluidic device and encapsulated in an array of individual microreactors, allowing for enzyme kinetics...
| Veröffentlicht in: | Langmuir : the ACS journal of surfaces and colloids. - 1992. - 24(2008), 9 vom: 06. Mai, Seite 4439-42 |
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| Format: | Online-Aufsatz |
| Sprache: | English |
| Veröffentlicht: |
2008
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| Zugriff auf das übergeordnete Werk: | Langmuir : the ACS journal of surfaces and colloids |
| Schlagworte: | Journal Article Galactosides Naphthoquinones Oxazines hydrolapachol 3343-38-2 resorufin galactopyranoside 95079-19-9 beta-Galactosidase EC 3.2.1.23 |
| Online verfügbar |
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