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231223s2008 xx |||||o 00| ||eng c |
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|a 10.1021/la703264x
|2 doi
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|a pubmed25n0595.xml
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|a eng
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| 100 |
1 |
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|a Averett, Laurel E
|e verfasserin
|4 aut
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| 245 |
1 |
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|a Complexity of "A-a" knob-hole fibrin interaction revealed by atomic force spectroscopy
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|c 2008
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
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|2 rdamedia
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|a Date Completed 11.06.2008
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|a Date Revised 29.04.2008
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a During blood vessel injury, fibrinogen is converted to fibrin, a polymer that serves as the structural scaffold of a blood clot. The primary function of fibrin is to withstand the large shear forces in blood and provide mechanical stability to the clot, protecting the wound. Understanding the biophysical forces involved in maintaining fibrin structure is of great interest to the biomedical community. Previous reports have identified the "A-a" knob-hole interaction as the dominant force responsible for fibrin's structural integrity. Herein, biochemical force spectroscopy is used to study knob-hole interactions between fibrin fragments and variant fibrinogen molecules to identify the forces occurring between individual fibrin molecules. The rupture of the "A-a" knob-hole interaction results in a characteristic profile previously unreported in fibrin force spectroscopy with two distinct populations of specific forces: 110 +/- 34 and 224 +/- 31 pN. In the absence of a functional "A" knob or hole "a", these forces cease to exist. We propose that the characteristic pattern represents the deformation of the D region of fibrinogen prior to the rupture of the "A-a" knob-hole bond
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Research Support, U.S. Gov't, Non-P.H.S.
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| 650 |
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|a Fibrin
|2 NLM
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| 650 |
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|a 9001-31-4
|2 NLM
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| 700 |
1 |
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|a Geer, Carri B
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Fuierer, Ryan R
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Akhremitchev, Boris B
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Gorkun, Oleg V
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Schoenfisch, Mark H
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Langmuir : the ACS journal of surfaces and colloids
|d 1991
|g 24(2008), 9 vom: 06. Mai, Seite 4979-88
|w (DE-627)NLM098181009
|x 0743-7463
|7 nnns
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| 773 |
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|g volume:24
|g year:2008
|g number:9
|g day:06
|g month:05
|g pages:4979-88
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|u http://dx.doi.org/10.1021/la703264x
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