Elicitor-dependent expression of the ribosome-inactivating protein beetin is developmentally regulated

BE27 and BE29 are two forms of beetin, a virus-inducible type 1 ribosome-inactivating protein isolated from leaves of Beta vulgaris L. Western blot analysis revealed the presence of beetin forms in adult plants but not in germ or young plants, indicating that the expression of these proteins is deve...

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Veröffentlicht in:Journal of experimental botany. - 1985. - 59(2008), 6 vom: 01., Seite 1215-23
1. Verfasser: Iglesias, Rosario (VerfasserIn)
Weitere Verfasser: Pérez, Yolanda, Citores, Lucía, Ferreras, José M, Méndez, Enrique, Girbés, Tomás
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2008
Zugriff auf das übergeordnete Werk:Journal of experimental botany
Schlagworte:Journal Article Research Support, Non-U.S. Gov't 3' Untranslated Regions Plant Proteins RNA, Messenger RNA, Plant Hydrogen Peroxide BBX060AN9V Ribosome Inactivating Proteins EC 3.2.2.22 mehr... Salicylic Acid O414PZ4LPZ
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520 |a BE27 and BE29 are two forms of beetin, a virus-inducible type 1 ribosome-inactivating protein isolated from leaves of Beta vulgaris L. Western blot analysis revealed the presence of beetin forms in adult plants but not in germ or young plants, indicating that the expression of these proteins is developmentally regulated. While beetins are expressed only in adult plants, their transcripts are present through all stages of development. In addition, the treatment of B. vulgaris leaves with mediators of plant-acquired resistance such as salicylic acid and hydrogen peroxide promoted the expression of beetin by induction of its transcript, but only in adult plants. The plant expresses three mRNAs which differ only in their 3' untranslated region. All these observations suggest a dual regulation of beetin expression, i.e. at the post-transcriptional and transcriptional levels. Additionally, total RNA isolated from leaves treated with hydrogen peroxide, which express high levels of active beetin, is not de-adenylated by endogenous beetin, nor in vitro by the addition of BE27, thus suggesting that sugar beet ribosomes are resistant to beetin 
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650 7 |a Salicylic Acid  |2 NLM 
650 7 |a O414PZ4LPZ  |2 NLM 
700 1 |a Pérez, Yolanda  |e verfasserin  |4 aut 
700 1 |a Citores, Lucía  |e verfasserin  |4 aut 
700 1 |a Ferreras, José M  |e verfasserin  |4 aut 
700 1 |a Méndez, Enrique  |e verfasserin  |4 aut 
700 1 |a Girbés, Tomás  |e verfasserin  |4 aut 
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