Two cDNA clones (FsDhn1 and FsClo1) up-regulated by ABA are involved in drought responses in Fagus sylvatica L. seeds
Two abscisic acid (ABA)-responsive clones (FsDhn1 and FsClo1) were isolated from a cDNA library of ABA-treated seeds of Fagus sylvatica L. FsDhn1 codes for type-II late embryogenesis abundant (LEA) proteins, also known as dehydrins. The corresponding transcripts were ABA-induced and expressed when s...
Veröffentlicht in: | Journal of plant physiology. - 1979. - 165(2008), 17 vom: 28. Nov., Seite 1798-807 |
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Weitere Verfasser: | , |
Format: | Online-Aufsatz |
Sprache: | English |
Veröffentlicht: |
2008
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Zugriff auf das übergeordnete Werk: | Journal of plant physiology |
Schlagworte: | Journal Article Research Support, Non-U.S. Gov't Calcium-Binding Proteins DNA, Complementary Plant Proteins RNA, Messenger late embryogenesis abundant protein, plant Water 059QF0KO0R Abscisic Acid mehr... |
Zusammenfassung: | Two abscisic acid (ABA)-responsive clones (FsDhn1 and FsClo1) were isolated from a cDNA library of ABA-treated seeds of Fagus sylvatica L. FsDhn1 codes for type-II late embryogenesis abundant (LEA) proteins, also known as dehydrins. The corresponding transcripts were ABA-induced and expressed when seeds were artificially dried. FsClo1 codes for a caleosin, a calcium-binding protein. By expressing FsCLO1 as a fusion protein in Escherichia coli, we obtained biochemical evidence of its ability to bind calcium, a second messenger involved in ABA signaling. This cDNA clone is seed specific, shows a LEA expression pattern and is also induced by ABA and dehydration treatments. The relationship of both genes with seed responses to ABA is discussed |
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Beschreibung: | Date Completed 10.06.2009 Date Revised 08.04.2022 published: Print-Electronic GENBANK: AJ585413, AJ606474 Citation Status MEDLINE |
ISSN: | 1618-1328 |
DOI: | 10.1016/j.jplph.2007.11.013 |