Calcium and magnesium cations enhance the adhesion of motile and nonmotile pseudomonas aeruginosa on alginate films

We investigated the impact of calcium and magnesium ions on the deposition kinetics of flagellated and nonflagellated Pseudomonas aeruginosa onto an alginate conditioning film in a radial stagnation point flow system. The bacterial deposition/adhesion behavior was related to structural changes of th...

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Veröffentlicht in:Langmuir : the ACS journal of surfaces and colloids. - 1992. - 24(2008), 7 vom: 01. Apr., Seite 3392-9
1. Verfasser: Kerchove, Alexis J de (VerfasserIn)
Weitere Verfasser: Elimelech, Menachem
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2008
Zugriff auf das übergeordnete Werk:Langmuir : the ACS journal of surfaces and colloids
Schlagworte:Journal Article Research Support, Non-U.S. Gov't Alginates Cations, Divalent Hexuronic Acids Glucuronic Acid 8A5D83Q4RW Magnesium I38ZP9992A Calcium SY7Q814VUP
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245 1 0 |a Calcium and magnesium cations enhance the adhesion of motile and nonmotile pseudomonas aeruginosa on alginate films 
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520 |a We investigated the impact of calcium and magnesium ions on the deposition kinetics of flagellated and nonflagellated Pseudomonas aeruginosa onto an alginate conditioning film in a radial stagnation point flow system. The bacterial deposition/adhesion behavior was related to structural changes of the alginate film in the presence of the divalent cations. Our results showed that adhesion of nonmotile bacteria was governed by cation bridging interactions between high-affinity sites at the bacterial surface and either clean or alginate-conditioned substrate surfaces. For motile bacteria, the adhesion onto clean quartz was governed by electrostatic interactions while adhesion onto alginate-conditioned quartz was dependent on the structure and viscoelastic properties of the alginate film in the presence of calcium or magnesium. We demonstrate that bacterial adhesion behavior is governed both by the effects of divalent cations on the surface properties of the bacteria and the substrate and by the type of specific interactions occurring between these two surfaces 
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650 4 |a Research Support, Non-U.S. Gov't 
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650 7 |a Calcium  |2 NLM 
650 7 |a SY7Q814VUP  |2 NLM 
700 1 |a Elimelech, Menachem  |e verfasserin  |4 aut 
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