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DE-627 |
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20250209055706.0 |
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231223s2008 xx ||||| 00| ||chi c |
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|a pubmed25n0592.xml
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|a (DE-627)NLM177663715
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|a (NLM)18279588
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|a DE-627
|b ger
|c DE-627
|e rakwb
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| 041 |
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|a chi
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| 100 |
1 |
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|a Fu, Yue-Qiang
|e verfasserin
|4 aut
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| 245 |
1 |
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|a Oxidative stress induced the expression of Bax and p53 during the apoptosis of alveolar type II epithelial cell
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| 264 |
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|c 2008
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|a Text
|b txt
|2 rdacontent
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|a ohne Hilfsmittel zu benutzen
|b n
|2 rdamedia
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| 338 |
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|a Band
|b nc
|2 rdacarrier
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| 500 |
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|a Date Completed 05.01.2010
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|a Date Revised 24.11.2016
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|a published: Print
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|a Citation Status MEDLINE
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|a OBJECTIVE: To investigate the effects of oxidative stress on the survival and apoptosis of alveolar epithelial type II (ATII) cells, as well as the mechanisms of apoptosis
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|a METHODS: 500 mumol/L H(2)O(2) was added into primary ATII cells at different times and cell viability, apoptotic ratio and the expression of Bax and p53 were measured by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, flow cytometry (FCM) and Western blotting analysis, respectively. The change in mitochondrial membrane potential (MMP) was detected by fluorescence microscopy and FCM
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|a RESULTS: The cell viability and MMP were decreased by H(2)O(2) compared with the controls (F(1)=85.211, F(2)=72.453, respectively, both P<0.05). The cell apoptotic ratios were increased with the time of the stimulation prolonged compared with the controls (F=54.002, P<0.05). H(2)O(2) increased Bax and p53 protein levels (F(1)=28.118, F(2)=43.456, both P<0.05)
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|a CONCLUSION: High level of oxidative stress can inhibit ATII cells proliferation, and induce cells apoptosis and decrease the MMP. Up-regulation of the expression of Bax and p53 may contribute to its apoptosis effects
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Bax protein, rat
|2 NLM
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| 650 |
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|a Tumor Suppressor Protein p53
|2 NLM
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| 650 |
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|a bcl-2-Associated X Protein
|2 NLM
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| 700 |
1 |
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|a Lu, Zhong-Yi
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Fang, Fang
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Tan, Li-Ping
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Kuang, Feng-Wu
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Wang, Xing-Yong
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Xu, Feng
|e verfasserin
|4 aut
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| 773 |
0 |
8 |
|i Enthalten in
|t Zhongguo wei zhong bing ji jiu yi xue = Chinese critical care medicine = Zhongguo weizhongbing jijiuyixue
|d 1998
|g 20(2008), 2 vom: 18. Feb., Seite 76-9
|w (DE-627)NLM098227793
|x 1003-0603
|7 nnns
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| 773 |
1 |
8 |
|g volume:20
|g year:2008
|g number:2
|g day:18
|g month:02
|g pages:76-9
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|a GBV_USEFLAG_A
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|a SYSFLAG_A
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|a GBV_NLM
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|a GBV_ILN_350
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|a AR
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| 952 |
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|d 20
|j 2008
|e 2
|b 18
|c 02
|h 76-9
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