Population study of sizes and components of self-reproducing giant multilamellar vesicles

Population analysis of a system of self-reproducing giant multilamellar vesicles (GMVs) was carried out by means of flow cytometry. The multidimensional distribution of forward light scattering (FS), side light scattering (SS), and fluorescence (FL) intensities originating from each GMV provided inf...

Description complète

Détails bibliographiques
Publié dans:Langmuir : the ACS journal of surfaces and colloids. - 1985. - 24(2008), 7 vom: 01. Apr., Seite 3037-44
Auteur principal: Toyota, Taro (Auteur)
Autres auteurs: Takakura, Katsuto, Kageyama, Yoshiyuki, Kurihara, Kensuke, Maru, Naoto, Ohnuma, Kiyoshi, Kaneko, Kunihiko, Sugawara, Tadashi
Format: Article en ligne
Langue:English
Publié: 2008
Accès à la collection:Langmuir : the ACS journal of surfaces and colloids
Sujets:Journal Article Research Support, Non-U.S. Gov't Liposomes
LEADER 01000caa a22002652c 4500
001 NLM177657723
003 DE-627
005 20250209055606.0
007 cr uuu---uuuuu
008 231223s2008 xx |||||o 00| ||eng c
024 7 |a 10.1021/la703017s  |2 doi 
028 5 2 |a pubmed25n0592.xml 
035 |a (DE-627)NLM177657723 
035 |a (NLM)18278955 
040 |a DE-627  |b ger  |c DE-627  |e rakwb 
041 |a eng 
100 1 |a Toyota, Taro  |e verfasserin  |4 aut 
245 1 0 |a Population study of sizes and components of self-reproducing giant multilamellar vesicles 
264 1 |c 2008 
336 |a Text  |b txt  |2 rdacontent 
337 |a ƒaComputermedien  |b c  |2 rdamedia 
338 |a ƒa Online-Ressource  |b cr  |2 rdacarrier 
500 |a Date Completed 05.05.2008 
500 |a Date Revised 25.03.2008 
500 |a published: Print-Electronic 
500 |a Citation Status MEDLINE 
520 |a Population analysis of a system of self-reproducing giant multilamellar vesicles (GMVs) was carried out by means of flow cytometry. The multidimensional distribution of forward light scattering (FS), side light scattering (SS), and fluorescence (FL) intensities originating from each GMV provided information about changes in a population composed of 104 vesicles. FS-FL dot plots indicated that, after the addition of the membrane precursor, the size distribution of the newly generated vesicles was nearly the same as that of the original, but the catalyst content was reduced. This result can be interpreted as evidence for the occurrence of the self-reproduction of GMVs. Moreover, the new GMVs recovered the amount of catalyst to the initial value, keeping their size distribution constant, when a solution of the catalyst was added to the new GMVs. These results are the first experimental evidence for a novel phenomenon on GMV size distribution during their self-reproducing cycle 
650 4 |a Journal Article 
650 4 |a Research Support, Non-U.S. Gov't 
650 7 |a Liposomes  |2 NLM 
700 1 |a Takakura, Katsuto  |e verfasserin  |4 aut 
700 1 |a Kageyama, Yoshiyuki  |e verfasserin  |4 aut 
700 1 |a Kurihara, Kensuke  |e verfasserin  |4 aut 
700 1 |a Maru, Naoto  |e verfasserin  |4 aut 
700 1 |a Ohnuma, Kiyoshi  |e verfasserin  |4 aut 
700 1 |a Kaneko, Kunihiko  |e verfasserin  |4 aut 
700 1 |a Sugawara, Tadashi  |e verfasserin  |4 aut 
773 0 8 |i Enthalten in  |t Langmuir : the ACS journal of surfaces and colloids  |d 1985  |g 24(2008), 7 vom: 01. Apr., Seite 3037-44  |w (DE-627)NLM098181009  |x 1520-5827  |7 nnas 
773 1 8 |g volume:24  |g year:2008  |g number:7  |g day:01  |g month:04  |g pages:3037-44 
856 4 0 |u http://dx.doi.org/10.1021/la703017s  |3 Volltext 
912 |a GBV_USEFLAG_A 
912 |a SYSFLAG_A 
912 |a GBV_NLM 
912 |a GBV_ILN_22 
912 |a GBV_ILN_350 
912 |a GBV_ILN_721 
951 |a AR 
952 |d 24  |j 2008  |e 7  |b 01  |c 04  |h 3037-44