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231223s2008 xx |||||o 00| ||eng c |
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|a 10.1093/jxb/erm354
|2 doi
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|a (NLM)18250077
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|a eng
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|a Bassel, George W
|e verfasserin
|4 aut
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|a Procera is a putative DELLA mutant in tomato (Solanum lycopersicum)
|b effects on the seed and vegetative plant
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|c 2008
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
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|2 rdamedia
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|a ƒa Online-Ressource
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|2 rdacarrier
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|a Date Completed 22.05.2008
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|a Date Revised 07.12.2022
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a The procera (pro) mutant of tomato exhibits a well-characterized constitutive gibberellic acid (GA) response phenotype. The tomato DELLA gene LeGAI in the pro mutant background contains a point mutation that results in an amino acid change in the conserved VHVID putative DNA-binding domain in LeGAI to VHEID. This same point mutation is in four different genetic backgrounds exhibiting the pro phenotype, suggesting that this mutation co-segregates with the pro phenotype. Complementation of the mutant with a constitutively expressed wild-type LeGAI gene sequence was not conclusive due to the infertility of transgenic plants. The pro mutation alters tomato branching architecture through differential suppression of axillary bud development, indicating a role for DELLA proteins in the regulation of plant structure. Isolated gib-1 pro double mutant embryo axes, which are unable to synthesize GA, germinate faster than their wild-type counterparts, and exert greater embryo growth potential. The pro mutation is therefore regulating GA responses within the tomato embryo. Transient expression of a LeGAI-GFP (green fluorescent protein) fusion protein in onion epidermis results in its location to the nucleus, and this protein is rapidly degraded by the proteasome in the presence of GA
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|a Journal Article
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|a Gibberellins
|2 NLM
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|a Plant Proteins
|2 NLM
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|a Green Fluorescent Proteins
|2 NLM
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|a 147336-22-9
|2 NLM
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|a gibberellic acid
|2 NLM
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|a BU0A7MWB6L
|2 NLM
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|a Proteasome Endopeptidase Complex
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|a Mullen, Robert T
|e verfasserin
|4 aut
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|a Bewley, J Derek
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Journal of experimental botany
|d 1985
|g 59(2008), 3 vom: 11., Seite 585-93
|w (DE-627)NLM098182706
|x 1460-2431
|7 nnns
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|g volume:59
|g year:2008
|g number:3
|g day:11
|g pages:585-93
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|u http://dx.doi.org/10.1093/jxb/erm354
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