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231223s2008 xx |||||o 00| ||eng c |
| 024 |
7 |
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|a 10.1021/la703053h
|2 doi
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|a pubmed24n1324.xml
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|a (NLM)18247639
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|a DE-627
|b ger
|c DE-627
|e rakwb
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| 041 |
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|a eng
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| 100 |
1 |
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|a Furbert, Patrick
|e verfasserin
|4 aut
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| 245 |
1 |
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|a Label-free optical detection of peptide synthesis on a porous silicon scaffold/sensor
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| 264 |
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|c 2008
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| 336 |
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
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|2 rdacarrier
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| 500 |
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|a Date Completed 14.05.2008
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|a Date Revised 12.03.2024
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a Mesoporous porous silicon (PSi) microcavity sensors are used to conduct conventional solid-phase peptide synthesis. The sensor optical response provides a convenient means to monitor the synthesis reaction in a nondestructive manner. Measurements indicate that peptide synthesis occurs only when the PSi sensor/scaffold is amine-terminated using, for example, the amino silane or deprotected acid-labile Rink linker. Equivalent coupling efficiencies of the first amino acid to both amine terminations are observed. Kinetic studies indicate that coupling reactions are 90% complete in 1 h. Quantitative analysis of the optical response following the synthesis of homo-oligopeptides (4-mers) suggests that coupling efficiencies and/or optical thickness changes depend on the peptide length. The synthesis of the cell adhesive oligopeptide (RGD) was monitored by the optical sensor response and validated by the cell culture of primary dermal fibroblasts. Secondary ion mass spectrometry (SIMS) analysis successfully detected peptide on the silicon wafer adjacent to the PSi. Our findings suggest the potential to exploit the high surface area, efficient coupling, and intrinsic optical detection properties of PSi for label-free high-throughput screening
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|a Journal Article
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|a Research Support, N.I.H., Extramural
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|a Amino Acids
|2 NLM
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| 650 |
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|a Oligopeptides
|2 NLM
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| 650 |
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|a arginyl-glycyl-aspartic acid
|2 NLM
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| 650 |
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|a 78VO7F77PN
|2 NLM
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| 650 |
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|a Silicon
|2 NLM
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| 650 |
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7 |
|a Z4152N8IUI
|2 NLM
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| 700 |
1 |
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|a Lu, Caiyan
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Winograd, Nicholas
|e verfasserin
|4 aut
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| 700 |
1 |
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|a DeLouise, Lisa
|e verfasserin
|4 aut
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| 773 |
0 |
8 |
|i Enthalten in
|t Langmuir : the ACS journal of surfaces and colloids
|d 1992
|g 24(2008), 6 vom: 18. März, Seite 2908-15
|w (DE-627)NLM098181009
|x 1520-5827
|7 nnns
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| 773 |
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|g volume:24
|g year:2008
|g number:6
|g day:18
|g month:03
|g pages:2908-15
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| 856 |
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|u http://dx.doi.org/10.1021/la703053h
|3 Volltext
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